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Sensitive and Selective LC–ESI–MS Analysis of Aesculin in Rat Plasma

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Abstract

A rapid and sensitive liquid chromatography–electrospray ionization mass spectrometry method was developed for the determination of aesculin in rat plasma. The analyses were chromatographed on a Zorbax Extend-C18 analytical column (150 × 2.1 mm I.D., 5 µm) with 30:70 (v/v) methanol–0.1% formic acid as mobile phase. Detection was performed by triple-quadrupole tandem mass spectrometry in multi-reaction-monitoring mode with an electrospray ionization source. The method was validated for accuracy and precision, and linearity in the two matrices was good. The assay was linear in the range 12.5–1,800 ng mL−1. The lower limit of quantification of aesculin (LLOQ) was 12.5 ng mL−1. The recovery of aesculin and tinidazole (IS) were well above 85%. The within- and between-batch accuracy was 100–104% and 97–109%, respectively. There were no stability-related problems in the procedure for the analysis of aesculin. The method was successfully used in a preclinical study of the pharmacokinetics of aesculin in rats.

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References

  1. Zhang HY, Li QF, Shi ZH, Hu ZD (2000) Talanta 52(4):607–621. doi:10.1016/S0039-9140(00)00343-X

    Article  CAS  Google Scholar 

  2. Wang CJ, Hsieh YJ, Chu CY, Lin YL, Tseng TH (2002) Cancer Lett 183(2):163–168

    Article  CAS  Google Scholar 

  3. Duncan SH, Flint HJ, Stewart CS (1998) FEMS Microbiol Lett 164(2):283–288

    Article  CAS  Google Scholar 

  4. Shi ZH (2007) J Pharm Biomed Anal 44(4):867–873. doi:10.1016/j.jpba.2007.03.035

    Article  CAS  Google Scholar 

  5. Zhang L, Tong P, Chen GN (2005) J Chromatogr A 1098(1–2):194–198. doi:10.1016/j.chroma.2005.10.029

    Article  CAS  Google Scholar 

  6. You TY, Yang XR, Wang EK (1999) Anal Chim Acta 401(1–2):29–34. doi:10.1016/S0003-2670(99)00497-3

    Article  CAS  Google Scholar 

  7. Yang XW, Xu B, Wu J, Wang RQ, Cui JR (2006) Modern Chin Med 81(2):108–114. doi:10.1016/j.yexmp.2006.06.003

    CAS  Google Scholar 

  8. Chen QH, Hou SX, Zheng J, Bi YQ, Yang XJ, Song XR (2007) J Chromatogr B 858(1–2):199–204. doi:10.1016/j.jchromb.2007.08.024

    Article  CAS  Google Scholar 

  9. Li CH, Chen AJ, Chen XF, Ma X, Chen XG, Hu ZD (2005) Biomed Chromatogr 19:696–702

    Article  CAS  Google Scholar 

  10. Li CH, Chen AJ, Chen XF, Chen XG, Hu ZD (2005) J Pharm Biomed Anal 39(1–2):125–131. doi:10.1016/j.jpba.2005.03.018

    Article  CAS  Google Scholar 

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Acknowledgments

This project was support by Key Technologies R&D Program of China (2006BAI14B07).

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Authors and Affiliations

  1. Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029, China

    Shijia Liu, Wenzheng Ju & Ningning Xiong

  2. Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029, China

    Xiaoyan Xia & Zixiu Liu

  3. General Hospital of Nanjing Military Base, Nanjing, Jiangsu, 210002, China

    Hengshan Tan

Authors
  1. Shijia Liu
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  2. Wenzheng Ju
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  3. Xiaoyan Xia
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  4. Zixiu Liu
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  5. Hengshan Tan
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  6. Ningning Xiong
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Correspondence to Shijia Liu.

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Liu, S., Ju, W., Xia, X. et al. Sensitive and Selective LC–ESI–MS Analysis of Aesculin in Rat Plasma. Chroma 70, 1121–1126 (2009). https://doi.org/10.1365/s10337-009-1306-6

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  • DOI: https://doi.org/10.1365/s10337-009-1306-6

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