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Quality Evaluation of Petasites tricholobus through Simultaneous Determination of Four Bioactive Bakkenolides by LC

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Abstract

A liquid chromatographic method was developed to evaluate the quality of Petasites tricholobus through a simultaneous determination of four major active bakkenolides. The wavelengths at 265 and 235 nm were chosen to determine four bakkenolides: bakkenolide-B, bakkenolide-D, bakkenolide-IIIa and bakkenolide-IVa. The recovery of the method was in the range of 98.6 to 103.1%, and all the bakkenolides showed good linearity (r 2 > 0.999) within test ranges. The developed method was applied to the determination of four bakkenolides in the collected herb samples. The results showed that the content of bakkenolides in rhizome was higher than in other parts of the plant and the older the rhizome, the higher was the bakkenolide content. This simple, rapid, low-cost and reliable LC-VWD method is suitable for routine quantitative analysis and quality control of petasites species plants.

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Acknowledgments

This study was supported by Science and Technology Commission Research Projects of Shanghai Municipality (08DZ19715).

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Authors and Affiliations

  1. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, No. 325, Guo-He Road, 200433, Shanghai, People’s Republic of China

    Yu-Liang Wang, Meili Guo & Yan Wang

  2. Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, 200240, Shanghai, People’s Republic of China

    Yu-Liang Wang

Authors
  1. Yu-Liang Wang
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  2. Meili Guo
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  3. Yan Wang
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Correspondence to Meili Guo.

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Wang, YL., Guo, M. & Wang, Y. Quality Evaluation of Petasites tricholobus through Simultaneous Determination of Four Bioactive Bakkenolides by LC. Chroma 70, 1367–1371 (2009). https://doi.org/10.1365/s10337-009-1305-7

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  • DOI: https://doi.org/10.1365/s10337-009-1305-7

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