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LC Analysis of Histamine and Other Biogenic Amines in Digesta Cultures

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Abstract

In the intestine, microbial protein degradation to histamine and other biologically active amines is believed to be relevant to the health of species. An LC method with fluorescence detection has been developed for analysis of histamine, phenylethylamine, isoamylamine, putrescine, cadaverine, tyramine, and spermidine. After pre-column derivatisation with 9-fluorenylmethyl chloroformate, amines were separated on a reversed-phase C18 polyamine column with a mobile phase gradient. Validation of the method included calibration experiments, determination of amine recovery, and repeatability tests. The method proved especially useful for detection of histamine, phenylethylamine, putrescine, and cadaverine at relevant concentrations in caecum cultures from horses. The limits of quantification for these four amines ranged between 80 nM (histamine) and 400 nM (phenylethylamine).

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Author notes

  1. Annette Zeyner

    Present address: Faculty of Agricultural and Environmental Sciences, Institute of Farm Animal Sciences and Technology, University of Rostock, Justus-von-Liebig-Weg 8, 18059, Rostock, Germany

Authors and Affiliations

  1. Institute of Animal Nutrition, Nutritional Diseases and Dietetics, University of Leipzig, Gustav-Kühn-Str. 8, 04159, Leipzig, Germany

    Wiebke Engelmann, Annette Zeyner & Klaus D. Markuske

  2. Institute for Veterinary Physiology, University of Leipzig, An den Tierkliniken 7, 04103, Leipzig, Germany

    Jörg R. Aschenbach

Authors
  1. Wiebke Engelmann
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  2. Annette Zeyner
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  3. Klaus D. Markuske
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  4. Jörg R. Aschenbach
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Correspondence to Jörg R. Aschenbach.

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Engelmann, W., Zeyner, A., Markuske, K.D. et al. LC Analysis of Histamine and Other Biogenic Amines in Digesta Cultures. Chroma 70, 1207–1213 (2009). https://doi.org/10.1365/s10337-009-1303-9

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  • DOI: https://doi.org/10.1365/s10337-009-1303-9

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