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LC with Fluorescence Detection for the Tissue Pharmacokinetics of 2-Methoxyestradiol Solution and Liposome After Intravenous Administrations to Rats

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Abstract

2-Methoxyestradiol is currently in phase II clinical trials as a chemotherapeutic agent. An LC method with fluorescence detection was developed for determination of methoxyestradiol in rat lung. Sample was extracted with ethyl acetate and separated on a C18 column (150 mm × 4.6 mm, 5 μm) with a mobile phase consisting of potassium dihydrogen phosphate-acetonitrile-triethylamine (55:45:0.3, v/v/v, pH 3.0). The excitation and emission wavelength were set at 285 and 325 nm, respectively. Standard curves were linear over the concentration range of 0.25–64 μg g−1. The method was found to be precise, accurate and specific and can be applied to tissue pharmacokinetics of methoxyestradiol in rats.

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Authors and Affiliations

  1. School of Pharmacy, Zhengzhou University, 450001, Zhengzhou, People’s Republic of China

    Bin Du, Ying Li, Xiaotian Li, Youmei A & Zhenzhong Zhang

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  1. Bin Du
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  2. Ying Li
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  3. Xiaotian Li
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  4. Youmei A
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  5. Zhenzhong Zhang
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Correspondence to Zhenzhong Zhang.

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Du, B., Li, Y., Li, X. et al. LC with Fluorescence Detection for the Tissue Pharmacokinetics of 2-Methoxyestradiol Solution and Liposome After Intravenous Administrations to Rats. Chroma 70, 1287–1290 (2009). https://doi.org/10.1365/s10337-009-1299-1

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  • DOI: https://doi.org/10.1365/s10337-009-1299-1

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