Abstract
Pindone is a highly effective anticoagulant rodenticide. In this paper, an improved assay for the analysis and confirmation of pindone in human plasma has been proposed. After the samples protein precipitation with 10% (v/v) methanol in acetonitrile and cleaning with solid-phase extraction, the separation was carried out on an IonPac AS11-HC analytical column (250 mm × 2 mm) using 20 mmol L−1 KOH containing 10% (v/v) methanol as organic modifier by eluent generator reagent free ion chromatography. Quantification was performed by a negative electrospray ionization ion trap mass spectrometry using diphacinone as an internal standard. The transition for quantitative analysis was m/z 229 → 172, and for qualitative analysis were m/z 229 → 145 and m/z 229 → 214 for pindone. The transition for quantitative analysis was m/z 339 → 167 for IS. The limit of detection, the limit of quantification, recovery, linearity, precision, and stability were well validated. The cracking approach of characteristic fragments for pindone and IS were proposed. It was confirmed that this method could be used in clinical diagnosis and forensic toxicology analysis.
References
Valchev I, Binev R, Yordanova V, Nikolov Y (2008) Turk J Vet Anim Sci 32:237–243
Beauregard JR, Tusing TW, Hanzal RF (1995) J Agric Food Chem 3:124–127. doi:10.1021/jf60048a002
Menzie CM, Adomaitis VA, Reichel WL (1962) Anal Chem 34:516–518. doi:10.1021/ac60184a021
Palazoglu MG, Tor ER, Holstege DM, Galey FD (1998) J Agric Food Chem 46:4260–4266. doi:10.1021/jf9803886
Marek LJ, Koskinen WC (2007) J Agric Food Chem 55:571–576. doi:10.1021/jf061440y
Jin MC, Chen XH (2006) Rapid Commun Mass Spectrom 20:2741–2746. doi:10.1002/rcm.2654
Ouyang XK, Jin MC, He CH (2007) Chromatographia 65:373–375. doi:10.1365/s10337-006-0156-8
Xu Q, Huang M, Jin MC, Ren QL (2007) Chromatographia 66:735–739. doi:10.1365/s10337-007-0403-7
Chen J, Paudel KS, Derbenev AV, Smith BN, Stinchcomb AL (2009) Chromatographia 69:1–7. doi:10.1365/s10337-008-0841-x
Gomes NA, Vaidya VV, Pudage AM, Joshi SS, Parekh SA, Tamhankar AV (2009) Chromatographia 69:19–25. doi:10.1365/s10337-008-0848-3
Cai MQ, Chen XH, OuYang XK (2009) Chromatographia 69:33–38. doi:10.1365/s10337-008-0844-7
Jin MC, Zhu Y (2006) J Chromatogr A 1118:111–117. doi:10.1016/j.chroma.2006.03.063
Meyer A, Höffler S, Fischer K (2007) J Chromatogr A 1170:62–72. doi:10.1016/j.chroma.2007.09.016
Xie R, Johnson W, Spayd S, Hall GS, Buckley B (2006) Anal Chim Acta 578:186–194. doi:10.1016/j.aca.2006.06.076
Jin MC, Chen XH, Ye ML, Zhu Y (2008) J Chromatogr A 1213:77–82. doi:10.1016/j.chroma.2008.08.100
Acknowledgements
This work was supported by the Natural Science Foundation of Ningbo, China (No. 2007A610072), the Agriculture and Social Development Funds of Ningbo, China (No. 2007C10084), the Medical Health Youth Funds in Zhejiang Province, China (No. 2007QN014), and the Medicine Science Foundation of Ningbo, Zhejiang Province, China (2008017).
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Chen, XH., Cai, MQ. & Jin, MC. Analysis and Confirmation of Rodenticide Pindone in Human Plasma by IC–ESI–IT–MS. Chroma 70, 1201–1206 (2009). https://doi.org/10.1365/s10337-009-1298-2
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DOI: https://doi.org/10.1365/s10337-009-1298-2