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Isolation of Terpenoids from Trichilia quadrijuga (Meliaceae) by Droplet Counter-Current Chromatography

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Abstract

The Trichilia genus (Meliaceae) consists of about 230 species distributed throughout tropical America and its phytochemical profile is rich in terpenic metabolites. Droplet counter-current chromatography (DCCC) was used for the isolation and purification of secondary metabolites obtained from a dichloromethane fraction (2.9 g) of Trichilia quadrijuga stems. A hexane–ethyl acetate–methanol–water (1:2:1.75:1, v/v/v/v) solvent system was chosen to isolate 2β,3β,4β-trihydroxypregnan-16-one (1, 24.5 mg, 0.8%), kudtdiol (2, 45.0 mg, 1.6%) and 3-O-β-d-glucopyranosylsitosterol (3, 6.0 mg, 0.2%). The results showed that DCCC was a very effective tool for the isolation of terpenes from T. quadrijuga.

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Acknowledgments

The authors are grateful to the technician Eliane Gonçalves de Carvalho from the Centro Analítico of Far-Manguinhos (Fiocruz) for the aqcisition of NMR spectra, the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ).

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Authors and Affiliations

  1. Laboratório de Tecnologia de Alimentos, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego 2000, Horto, Campos dos Goytacazes, Rio de Janeiro, 28013-602, Brazil

    Virginia Freitas Rodrigues & Ivo José C. Vieira

  2. Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego 2000, Horto, Campos dos Goytacazes, Rio de Janeiro, 28013-602, Brazil

    Virginia Freitas Rodrigues, Hadria M. Carmo, Rodrigo R. Oliveira, Raimundo Braz Filho, Leda Mathias & Ivo José C. Vieira

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  1. Virginia Freitas Rodrigues
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  2. Hadria M. Carmo
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  3. Rodrigo R. Oliveira
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  4. Raimundo Braz Filho
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  5. Leda Mathias
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  6. Ivo José C. Vieira
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Correspondence to Virginia Freitas Rodrigues.

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Rodrigues, V.F., Carmo, H.M., Oliveira, R.R. et al. Isolation of Terpenoids from Trichilia quadrijuga (Meliaceae) by Droplet Counter-Current Chromatography. Chroma 70, 1191–1195 (2009). https://doi.org/10.1365/s10337-009-1293-7

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  • DOI: https://doi.org/10.1365/s10337-009-1293-7

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