Abstract
Ion-interaction chromatography with direct conductivity detection has been used for analysis of nitrate and nitrite. Chromatographic separation was performed on a monolithic silica-based C18 column dynamically modified with tetrabutylammonium (TBA+). Using the optimized mobile phase, containing 2.0 mmol L−1 TBA+ and 0.8 mmol L−1 citrate (pH 6.0), delivered at a flow rate of 6.0 mL min−1, separation of five anions (chloride, nitrite, bromide, nitrate, and sulfate) was achieved in only 40 s at a column temperature of 30 °C. The detection limits for nitrate and nitrite were 0.74 and 0.92 mg L−1, respectively. The relative standard deviation (RSD, n = 5) of the retention times of nitrate and nitrite was 0.1% and RSD of chromatographic peak areas were 0.4 and 0.2%, respectively. The method was successfully used for analysis of the anions in groundwater. Recovery of nitrate and nitrite was 99.1 and 105%, respectively.
Similar content being viewed by others
References
Weisburger JH (1986) Nutr Cancer 8:47–62. PMID:3012485
Ellis G, Adatia I, Yazdanpanah M, Makela SK (1998) Clin Biochem 31:195–220. doi:10.1016/S0009-9120(98)00015-0
Suzuki T, Inukai M (2006) Chem Res Toxicol 19:457–462. doi:10.1021/tx050347l
Connolly D, Paull B (2001) J Chromatogr A 917:353–359. doi:10.1016/S0021-9673(01)00686-0
Connolly D, Paull B (2001) Anal Chim Acta 441:53–62. doi:10.1016/S0003-2670(01)01068-6
Connolly D, Paull B (2002) J Chromatogr A 953:299–303. doi:10.1016/S0021-9673(02)00108-5
Zuo YG, Wang CJ, Van T (2006) Talanta 70:281–285. doi:10.1016/j.talanta.2006.02.034
Zhu Y, Ling YY, James SF, Haddad PR (2003) J Chromatogr A 1020:259–264. doi:10.1016/j.chroma.2003.08.009
Okemgbo AA, Hill HH, Siems WF, Metcalf SG (1999) Anal Chem 71:2725–2731. doi:10.1021/ac990198+
Woodland MA, Lucy CA (2001) Analyst 126:28–32. doi:10.1039/b007900f
Hjerten S, Liao JL, Zhang R (1989) J Chromatogr A 473:273–275. doi:10.1016/S0021-9673(00)91309-8
Minakuchi H, Nakanishi K, Soga N, Ishizuka N, Tanaka N (1996) Anal Chem 68:3498–3501. doi:10.1021/ac960281m
Zou HF, Huang XD, Ye ML, Luo QZ (2002) J Chromatogr A 954:5–23. doi:10.1016/S0021-9673(02)00072-9
Siouffi AM (2003) J Chromatogr A 1000:801–818. doi:10.1016/S0021-9673(03)00510-7
Wu RA, Hu LH, Wang FJ, Ye ML, Zou HF (2008) J Chromatogr A 1184:369–392. doi:10.1016/j.chroma.2007.09.022
Zakaria P, Hutchinson JP, Avdalovic N, Liu Y, Haddad PR (2005) Anal Chem 77:417–423. doi:10.1021/ac048747l
Paull B, Nesterenko P (2005) Trends Anal Chem 24:295–303. doi:10.1016/j.trac.2004.11.018
Schaller D, Hilder EF, Haddad PR (2006) J Sep Sci 29:1705–1719. doi:10.1002/jssc.200600169
Chambers SD, Glenn KM, Lucy CA (2007) J Sep Sci 30:1628–1645. doi:10.1002/jssc.200700090
Ito K, Takayama Y, Makabe N, Mitsui R, Hirokawa T (2005) J Chromatogr A 1083:63–67. doi:10.1016/j.chroma.2005.05.073
Sugrue E, Nesterenko PN, Paull B (2005) J Chromatogr A 1075:167–175. doi:10.1016/j.chroma.2005.03.126
Victory D, Nesterenko P, Paull B (2004) Analyst 129:700–701. doi:10.1039/b407483a
Pelletier S, Lucy CA (2006) J Chromatogr A 1118:12–18. doi:10.1016/j.chroma.2006.03.072
Suzuki A, Lim LW, Hiroi T, Takeuchi T (2006) Talanta 70:190–193. doi:10.1016/j.talanta.2005.12.064
Hatsis P, Lucy CA (2002) Analyst 127:451–454. doi:10.1039/b111451d
Zhou S, Yu H, Yang L, Ai HJ (2008) J Chromatogr A 1206:200–203. doi:10.1016/j.chroma.2008.08.048
Yu H, Li RS (2008) Chin J Anal Chem 36(6):835–838
Ai HJ, Yu H, Zhou S, Li RS (2008) J Instrum Anal 27(9):991–993
Acknowledgments
This work was supported by Harbin Normal University (grant no. 08XYG-15).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yu, H., Ai, H. & Chen, Q. Rapid Analysis of Nitrate and Nitrite by Ion-Interaction Chromatography on a Monolithic Column. Chroma 70, 1017–1022 (2009). https://doi.org/10.1365/s10337-009-1292-8
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-009-1292-8