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Enantioseparation of Dioxopromethazine Hydrochloride in Urine with Liquid–Liquid Extraction by CE-ECL Detection

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Abstract

Capillary electrophoresis coupled with electrochemiluminescence detection was developed for the separation and determination of dioxopromethazine hydrochloride (DPZ) enantiomers. Performance parameters of the proposed method were evaluated. An improved separation of DPZ enantiomers could be achieved after adding boric acid to buffer. The enantiomers were completely separated with running buffer of 16.5 mM β-CD in 25 mM tris-H3PO4–40 mM H3BO3 at pH 2.5. The proposed method was successfully applied to the separation and determination of DPZ enantiomers in human urine with a liquid–liquid extraction procedure.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 20605020).

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Authors and Affiliations

  1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China

    Xia Li, Baiqing Yuan, Jinying Sun & Tianyan You

  2. Changchun University of Science and Technology, Changchun, Jilin, 130022, China

    Xia Li & Bo Song

Authors
  1. Xia Li
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  2. Bo Song
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  3. Baiqing Yuan
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  4. Jinying Sun
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  5. Tianyan You
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Correspondence to Tianyan You.

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Li, X., Song, B., Yuan, B. et al. Enantioseparation of Dioxopromethazine Hydrochloride in Urine with Liquid–Liquid Extraction by CE-ECL Detection. Chroma 70, 1291–1293 (2009). https://doi.org/10.1365/s10337-009-1287-5

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  • DOI: https://doi.org/10.1365/s10337-009-1287-5

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