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Study on Absorption of Radix Astragali in Rabbit Plasma by Alternative Moving Window Factor Analysis and LC–DAD–MS

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Abstract

In the present paper, study on the multiple absorbed bioactive components of Radix Astragali in rabbit was performed by alternative moving window factor analysis (AMWFA) and LC–DAD–MS. AMWFA was used to confirm the common components both in its abstract fingerprint and plasma fingerprint. This method could be utilized not only to determine the number of common components in different systems but also to identify their corresponding spectra. As can be seen from the results, the method determines the common components in different chromatographic fingerprints more efficient and reliable, especially for none or only weak selective information of some chromatographic peak clusters. With the aid of AMWFA both its abstract fingerprint and plasma fingerprint, one could find that 25 chemical components in RA were absorbed into the rabbit body after oral solution of RA administration.

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Acknowledgments

The educational department of Liaoning Province (No. 2008174) financially supported this research work. All the authors express their deep thanks.

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Authors and Affiliations

  1. Department of Laboratory Diagnostic Medicine, Dalian Medical University, 116044, Dalian, People’s Republic of China

    Ping Wang

  2. College of Chemistry and Chemical Engineering, Research Center of Modernization of Chinese Medicines, Central South University, 410083, Changsha, People’s Republic of China

    Ping Wang & Yizeng Liang

  3. Analytical Testing Center of Xiangya Medical College, Central South University, 410078, Changsha, People’s Republic of China

    Benmei Chen

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  1. Ping Wang
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  2. Yizeng Liang
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Correspondence to Ping Wang.

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Wang, P., Liang, Y. & Chen, B. Study on Absorption of Radix Astragali in Rabbit Plasma by Alternative Moving Window Factor Analysis and LC–DAD–MS. Chroma 70, 1245–1251 (2009). https://doi.org/10.1365/s10337-009-1285-7

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  • DOI: https://doi.org/10.1365/s10337-009-1285-7

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