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Simultaneous Determination of Main Bioactive Components in Rosa multiflora Thunb. and Their Fragmentation Study by LC–MS

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Abstract

Five naturally bioactive compounds, belonging to the representative flavonoid and pentacyclic triterpenoid types, were separated by liquid chromatography and analyzed on-line with negative ion ESI–MS. Rutin, quercetin, and oleanolic acid in the extracts of Rosa multiflora Thunb. of China were identified by the comparison of their retention times and mass spectrometric data with those of authentic standards, and their contents were 0.22, 3.8, and 45 µg g−1 of dry weight in rose hips, respectively. Five standards, including rutin, quercetin, kaempferol, lysionotin, and oleanolic acid were investigated by MS3, which facilitate further understanding of their structures.

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References

  1. Gao XM, Xu ZM, Li ZW (2000) Traditional Chinese medicines. People’s Health Publishing House, Beijing, p 2019

    Google Scholar 

  2. Li YF, Hu LH, Lou FC (2003) Chin Pharm J 38:336–338

    Google Scholar 

  3. Kumar N, Bhandari P, Singh B (2008) J Sep Sci 31:262–267. doi:10.1002/jssc.200700372

    Article  CAS  Google Scholar 

  4. Şakiroğlu H, Küfrevioğlu ÖI, Kocaçalişkan I, Oktay M, Onganer Y (1996) J Agric Food Chem 44:2982–2986. doi:10.1021/jf950808g

    Article  Google Scholar 

  5. Bhandari P, Kumar N, Gupta AP, Singh B, Kaul VK (2007) J Sep Sci 30:2092–2096. doi:10.1002/jssc.200700066

    Article  CAS  Google Scholar 

  6. Velioglu YS, Mazza G (1991) J Agric Food Chem 39:463–467. doi:10.1021/jf00003a007

    Article  CAS  Google Scholar 

  7. Knapp H, Straubinger M, Fornart S, Oka N, Watanabe N, Winterhalter P (1998) J Agric Food Chem 46:1966–1970. doi:10.1021/jf970987x

    Article  CAS  Google Scholar 

  8. Oka N, Ikegami A, Ohki M, Sakata K, Yagi A, Watanabe N (1998) Phytochemistry 47:1527–1529. doi:10.1016/S0031-9422(97)00526-8

    Article  CAS  Google Scholar 

  9. Okuda T, Yoshida T, Hatano T, Iwasaki M, Kubo M, Orime T, Yoshizaki M, Naruhashi N (1992) Phytochemistry 31:3091–3096. doi:10.1016/0031-9422(92)83451-4

    Article  CAS  Google Scholar 

  10. Mikanagi Y, Yokoi M, Ueda Y, Saito N (1995) Biochem Syst Ecol 23:183–200. doi:10.1016/0305-1978(95)93849-X

    Article  CAS  Google Scholar 

  11. Hashidoko Y (1996) Phytochemistry 43:535–549. doi:10.1016/0031-9422(96)00287-7

    Article  CAS  Google Scholar 

  12. Mikanagi Y, Saito N, Yokoi M, Tatsuzawa F (2000) Biochem Syst Ecol 28:887–902. doi:10.1016/S0305-1978(99)00127-1

    Article  CAS  Google Scholar 

  13. Cai YZ, Xing J, Sun M, Zhan ZQ, Corke H (2005) J Agric Food Chem 53:9940–9948. doi:10.1021/jf052137k

    Article  CAS  Google Scholar 

  14. Hvattum E (2002) Rapid Commun Mass Spectrom 16:655–662. doi:10.1002/rcm.622

    Article  CAS  Google Scholar 

  15. Hornero-Méndez D, Mínguez-Mosquera MI (2000) J Agric Food Chem 48:825–828. doi:10.1021/jf991136n

    Article  Google Scholar 

  16. Strålsjö L, Alkkint C, Olsson ME, Sjöholm I (2003) J Agric Food Chem 51:4291–4295. doi:10.1021/jf034208q

    Article  Google Scholar 

  17. Zhang JM, Satterfield MB, Brodbelt JS, Britz SJ, Clevidence B, Novotny JA (2003) Anal Chem 75:6401–6407. doi:10.1021/ac034795e

    Article  CAS  Google Scholar 

  18. Wittig J, Herderich M, Graefe EU, Veit M (2001) J Chromatogr B 753:237–243. doi:10.1016/S0378-4347(00)00549-1

    Article  CAS  Google Scholar 

  19. Schieber A, Berardini N, Carle R (2003) J Agric Food Chem 51:5006–5011. doi:10.1021/jf030218f

    Article  CAS  Google Scholar 

  20. Liu Y, Wagner H, Bauer R (1996) Phytochemistry 42:1203–1205. doi:10.1016/0031-9422(96)00066-0

    Article  CAS  Google Scholar 

  21. Song M, Hang TJ, Wang Y, Jiang L, Wu XL, Zhang ZX, Shen JP, Zhang YD (2006) J Pharm Biomed Anal 40:190–196. doi:10.1016/j.jpba.2005.06.034

    Article  CAS  Google Scholar 

  22. Ma YL, Li QM, Van den Heuvel H, Claeys M (1997) Rapid Commun Mass Spectrom 11:1357–1364. doi:10.1002/(SICI)1097-0231(199708)11:12<1357::AID-RCM983>3.0.CO;2-9

    Article  CAS  Google Scholar 

  23. Justesen U, Arrigoni E (2001) Rapid Commun Mass Spectrom 15:477–483. doi:10.1002/rcm.250

    Article  CAS  Google Scholar 

  24. Fabre N, Rustan I, Hoffmann E, Quetin-Leclercq J (2001) J Am Soc Mass Spectrom 12:707–715. doi:10.1016/S1044-0305(01)00226-4

    Article  CAS  Google Scholar 

  25. Hvattum E, Ekeberg D (2003) J Mass Spectrom 38:43–49. doi:10.1002/jms.398

    Article  CAS  Google Scholar 

  26. Justesen U (2000) J Chromatogr A 902:369–379. doi:10.1016/S0021-9673(00)00861-X

    Article  CAS  Google Scholar 

  27. Justino GC, Borges CM, Florêncio MH (2009) Rapid Commun Mass Spectrom 23:237–248. doi:10.1002/rcm.3869

    Article  CAS  Google Scholar 

  28. Justesen U (2001) J Mass Spectrom 36:169–178. doi:10.1002/jms.118

    Article  CAS  Google Scholar 

  29. van der Doelen GA, van den Berg KJ, Boon JJ, Shibayama N, de la Rie ER, Genuit WJL (1998) J Chromatogr A 809:21–37. doi:10.1016/S0021-9673(98)00186-1

    Article  Google Scholar 

  30. Modugno F, Ribechini E, Colombini MP (2006) Rapid Commun Mass Spectrom 20:1787–1800. doi:10.1002/rcm.2507

    Article  CAS  Google Scholar 

  31. Matuszewski BK, Constanzer ML, Chavez-Eng CM (2003) Anal Chem 75:3019–3030. doi:10.1021/ac020361s

    Article  CAS  Google Scholar 

  32. Matuszewski BK, Constanzer ML, Chavez-Eng CM (1998) Anal Chem 70:882–889. doi:10.1021/ac971078+

    Article  CAS  Google Scholar 

  33. Matuszewski BK (2006) J Chromatogr B 830:293–300. doi:10.1016/j.jchromb.2005.11.009

    Article  CAS  Google Scholar 

  34. Constanzer ML, Chavez-Eng CM, Fu I, Woolf EJ, Matuszewski BK (2005) J Chromatogr B 816:297–308. doi:10.1016/j.jchromb.2004.11.049

    Article  CAS  Google Scholar 

  35. Constanzer M, Chavez-Eng C, Matuszewski B (2001) J Chromatogr B 760:45–53. doi:10.1016/S0378-4347(01)00248-1

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This work was supported by NSFC (Grant no. 20865004).

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Authors and Affiliations

  1. Beijing National Laboratory for Molecular Sciences, the Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, 100871, Beijing, People’s Republic of China

    Nannan Pang & Huwei Liu

  2. College of Life and Environmental Sciences, Xinjiang Normal University, 830053, Xinjiang, People’s Republic of China

    Dilinur Malike

Authors
  1. Nannan Pang
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  2. Dilinur Malike
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  3. Huwei Liu
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Correspondence to Dilinur Malike or Huwei Liu.

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Pang, N., Malike, D. & Liu, H. Simultaneous Determination of Main Bioactive Components in Rosa multiflora Thunb. and Their Fragmentation Study by LC–MS. Chroma 70, 1253–1257 (2009). https://doi.org/10.1365/s10337-009-1281-y

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  • DOI: https://doi.org/10.1365/s10337-009-1281-y

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