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Rapid Separation of Hydrophobic Compounds by UPLC: in Case of Bisindolylmaleimides, Indolocarbazoles or Fullerene C60 Derivatives

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Abstract

Rapid separation of hydrophobic compounds such as bisindolylmaleimides, indolocarbazoles or fullerene C60 derivatives by ultra performance liquid chromatography was developed. Bisindolylmaleimides and indolocarbazoles were separated within 7 and 5 min, respectively. The linear ranges for the determination of bisindolylmaleimides and indolocarbazoles were 1.25−500 pmol and 0.5−50 pmol per injection (r > 0.99), respectively. Fullerene C60 derivatives were separated within 9 min, and the linear range for the determination of fullerene C60 derivatives was 0.1−10 pmol per injection (r > 0.99).

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Acknowledgments

We gratefully thank Waters (Tokyo, Japan) for the use of the Acquity UPLC system in the university program.

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Manabu Nakazono, Yasuyuki Sato, Shigeo Hasegawa, Norimasa Arisuda, Aiko Miyamoto & Kiyoshi Zaitsu

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  1. Manabu Nakazono
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  2. Yasuyuki Sato
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  3. Shigeo Hasegawa
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  4. Norimasa Arisuda
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  5. Aiko Miyamoto
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  6. Kiyoshi Zaitsu
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Correspondence to Kiyoshi Zaitsu.

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Nakazono, M., Sato, Y., Hasegawa, S. et al. Rapid Separation of Hydrophobic Compounds by UPLC: in Case of Bisindolylmaleimides, Indolocarbazoles or Fullerene C60 Derivatives. Chroma 70, 933–937 (2009). https://doi.org/10.1365/s10337-009-1271-0

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  • DOI: https://doi.org/10.1365/s10337-009-1271-0

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