Abstract
As part of our ongoing studies, a new and validated method for analysis of isohexenyl-naphthazarines in Turkish Alkanna species was developed. By using reversed phase material (Synergi MAX RP) it was possible to determine four known isohexenylnaphthazarin-derivatives, namely alkannin, acetylalkannin, deoxyalkannin, and β,β-dimethylacrylalkannin within 25 min in n-hexane extracts of the roots. The quantification of these compounds in 16 Anatolian Alkanna species is described for the first time. In order to assure peak identity LC-MS experiments were performed. Quantitative results revealed that the most dominant compound in the extracts was β,β-dimethylacrylalkannin.
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Akgun, I., Ganzera, M., Gur, C. et al. Determination of Naphthazarin Derivatives in 16 Alkanna Species by RP-LC Using UV and MS for Detection. Chroma 70, 963–967 (2009). https://doi.org/10.1365/s10337-009-1261-2
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DOI: https://doi.org/10.1365/s10337-009-1261-2