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LC Analysis of Aliphatic Primary Amines and Diamines After Derivatization with 2,6-Dimethyl-4-quinolinecarboxylic Acid N-Hydroxysuccinimide Ester

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Abstract

A method for sensitive simultaneous analysis of aliphatic primary amines and diamines has been developed and validated. The compounds were analyzed by reversed-phase high-performance liquid chromatography after pre-column derivatization with 2,6-dimethyl-4-quinolinecarboxylic acid N-hydroxysuccinimide ester as fluorescent probe. The derivatization reaction was performed at 50 °C for 40 min in 0.1 mol L−1 borax buffer solution (pH 7.5). The resulting fluorophores were separated to baseline on a C18 column and fluorimetrically detected at λ ex/λ em = 326/409 nm. Detection limits were in the range 0.50–0.02 nmol L−1. The method was successfully used for analysis of aliphatic amines in water, human urine, and serum.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (20805040), the Natural Science Foundation of He’nan Province of China (082300420140), the Foundation of He’nan Educational Committee (2008A150020), China Postdoctoral Science Foundation Funded project (20080430163), Jiangsu planned projects for postdoctoral research funds (0801041B), and Excellent Youth Foundation of He’nan Scientific Committee (084100410024).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Xinyang Normal University, 464000, Xinyang, People’s Republic of China

    Ke-Jing Huang, Cai-Yun Wei, Wan-Zhen Xie & Yan-Ming Liu

  2. Xinyang Central Hospital, 464000, Xinyang, People’s Republic of China

    Yong-Hong Chen

  3. Research Center for Agricultural Standards and Inspection Technology, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, People’s Republic of China

    Jun-Feng Zhang

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  1. Ke-Jing Huang
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Correspondence to Ke-Jing Huang.

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Huang, KJ., Wei, CY., Xie, WZ. et al. LC Analysis of Aliphatic Primary Amines and Diamines After Derivatization with 2,6-Dimethyl-4-quinolinecarboxylic Acid N-Hydroxysuccinimide Ester. Chroma 70, 747–752 (2009). https://doi.org/10.1365/s10337-009-1256-z

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