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Biopartitioning Micellar Chromatography to Predict Dihydropyridine Selective Calcium Channel Antagonist Toxicity

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Abstract

With the accelerating development of new drugs, there is a great need for rapid and simple screening technologies for estimating the potential toxicity of new compounds. LD50 determination is the main way to measure the acute toxicity of all types of substances. In this paper, a new in vitro method, biopartitioning micellar chromatography, was developed for predicting oral acute toxicity (LD50) based on the similar properties between biopartitioning micellar chromatography systems and biological barriers and extracellular fluids. A second-order polynomial model (an LD50 model) has been obtained using the retention data of the dihydropyridine selective calcium channel antagonists to predict the pharmacological toxicity of the same compounds.

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Acknowledgments

This work is supported by the Natural Science Foundation, Hebei Province (C200700082), the backbone of the scientific research training program, Hebei Medical University (200701).

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Authors and Affiliations

  1. Pharmacology Department, Basic Medical College, Hebei Medical University, Zhongshan East Road 361, 050017, Shijiazhuang, China

    Ning Zhang, Wenwen Che, Sijia Xu & Sumin Wang

  2. College of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Yuhua East Road 70, 050018, Shijiazhuang, China

    Zhiwei Li

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  1. Ning Zhang
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  2. Zhiwei Li
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  3. Wenwen Che
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  4. Sijia Xu
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  5. Sumin Wang
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Correspondence to Sumin Wang.

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Zhang, N., Li, Z., Che, W. et al. Biopartitioning Micellar Chromatography to Predict Dihydropyridine Selective Calcium Channel Antagonist Toxicity. Chroma 70, 685–690 (2009). https://doi.org/10.1365/s10337-009-1251-4

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  • DOI: https://doi.org/10.1365/s10337-009-1251-4

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