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Rapid Determination of Cichoric Acid by Argentation Complex Chromatography and Study on Related Mechanisms of Coordination Simultaneously Used in Rapid Determination of Salvianolic Acid B

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Abstract

Cichoric acid is a water-soluble phenolic acid in Echinacea purpurea and has a high medicinal value. A rapid and novel argentation complex liquid chromatography method has been developed and validated for determination of cichoric acid in E. purpurea extract. The determination of cichoric acid was carried out on a Restek Pinnacle 11 C18 column (250 mm × 4.6 mm, 5 μm), using acetonitrile-deionized water (38:62, v/v, with 6 mmol L−1 AgNO3 and 0.8% acetic acid) as the mobile phase at a flow rate of 1.0 mL min−1 within 10 min. The wavelength was set at 326 nm. It improved the effect of determination by adding AgNO3 in the mobile phase while cichoric acid coordinated with Ag+. The mechanism of coordination between cichoric acid and Ag+ has been studied by Fourier transform infrared spectroscopy, electrospray ionization mass spectrometry. The mechanism which improved the effect of determination of cichoric acid is analyzed and applied in the rapid determination of salvianolic acid B (Sal B) in Danshen extract solution which has been stored for half a year. The possible structures of the complex and complex ratio are all provided in this article. The experiments have facilitated the study of cichoric acid–Ag+ and Sal B–Ag+ complex and provide a theoretical basis for industrialized extraction of cichoric acid and Sal B in the future.

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Acknowledgments

This work has been supported by Grant 20136020 from China National Natural Science Foundation.

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Authors and Affiliations

  1. Engineering Research Center of Bio-Process, Hefei University of Technology, Ministry of Education, 230009, Hefei, Anhui, People’s Republic of China

    Beibei Zhou & Jian Pan

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  1. Beibei Zhou
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  2. Jian Pan
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Correspondence to Beibei Zhou.

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Zhou, B., Pan, J. Rapid Determination of Cichoric Acid by Argentation Complex Chromatography and Study on Related Mechanisms of Coordination Simultaneously Used in Rapid Determination of Salvianolic Acid B. Chroma 70, 739–745 (2009). https://doi.org/10.1365/s10337-009-1246-1

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