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Sensitive Quantification of Carboxylic Acid Metabolite of Clopidogrel in Human Plasma by LC with UV Detection

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Abstract

A rapid LC method with UV detection was developed for the quantification of carboxylic acid metabolite of clopidogrel in human plasma. Following a simple protein precipitation using a mixture of methanolic solution of ZnSO4, the analyte and commercially available internal standard were separated using a mobile phase of water–acetonitril (85:15, v/v) adjusted to pH 3.5 on a Chromolith C18 column at a flow rate of 2.5 mL min−1 with a total retention time of 4 min. Linearity was verified over the range of 20–3,000 ng mL−1 where the LOQ was 20 ng mL−1. This method was applied in a pharmacokinetic study.

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References

  1. PLAVIXTM, RxMed: Pharmaceutical information, Sanofi/Bristol-Mayers Squibb

  2. Pereillo JM, Maftouh M, Andrieu A, Uzabiaga MF, Fedeli O, Savi P, Pascal M, Herbert JM, Maffrand JP, Picard C (2002) Drug Metab Dispos 30:1288–1295. doi:10.1124/dmd.30.11.1288

    Article  CAS  Google Scholar 

  3. Lagorce P, Perez Y, Ortiz J, Necciari J, Bressole F (1998) J Chromatogr B Analyt Technol Biomed Life Sci 720:107–117. doi:10.1016/S0378-4347(98)00452-6

    Article  CAS  Google Scholar 

  4. Singh SS, Sharma K, Barot D, Mohan PR, Lohray VB (2005) J Chromatogr B Analyt Technol Biomed Life Sci 821:173–180. doi:10.1016/j.jchromb.2005.05.013

    Article  CAS  Google Scholar 

  5. Nirogi RV, Kandikere VN, Shukla M, Mudigonda K, Maurya S, Boosi R (2006) Rapid Commun Mass Spectrom 20:1695–1700. doi:10.1002/rcm.2497

    Article  CAS  Google Scholar 

  6. Robinson A, Hillis J, Neal C, Leary AC (2007) J Chromatogr B Analyt Technol Biomed Life Sci 848:344–354. doi:10.1016/j.jchromb.2006.10.076

    Article  CAS  Google Scholar 

  7. Bahrami G, Mohammadi B, Sisakhtnezhad S (2008) J Chromatogr B Analyt Technol Biomed Life Sci 864:168–172. doi:10.1016/j.jchromb.2008.01.049

    Article  CAS  Google Scholar 

  8. Souri E, Jalalizadeh H, Kebriaee-Zadeh A, Shekarchi M, Dalvandi A (2006) Biomed Chromatogr 20:1309–1314. doi:10.1002/bmc.697

    Article  CAS  Google Scholar 

  9. Guidance for Industry Bioanalytical Method Validation, US Department of Health and Human Services Food and Drug Administration, Center for Drug Evaluation and Research (CDER) (2001)

  10. Mani H, Toennes SW, Linnemann B, Urbanek DA, Schwonberg J, Kauert GF, Lindhoff-Last E (2008) Ther Drug Monit 30:84–89. doi:10.1097/FTD.0b013e31815c13fd

    Article  CAS  Google Scholar 

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Acknowledgments

This work was fully supported by a grant from the Pharmaceutical Sciences Research Centre, Tehran University of Medical Sciences. The authors wish to thank Osvah Pharmaceutical Co. for their kind donation of CCA.

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Authors and Affiliations

  1. Biopharmaceutics and Pharmacokinetics Division, Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 14155-6451, Iran

    Mohammad-Reza. Rouini, Yalda H. Ardakani, Hoda Lavasani & Lida Hakemi

  2. Faculty of Pharmacy, Pharmaceutical Sciences Research Centre, Tehran University of Medical Sciences, Tehran, 14155-6451, Iran

    Mohammad-Reza. Rouini & Alireza Foroumadi

Authors
  1. Mohammad-Reza. Rouini
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  2. Yalda H. Ardakani
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  3. Alireza Foroumadi
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  4. Hoda Lavasani
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  5. Lida Hakemi
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Correspondence to Mohammad-Reza. Rouini.

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Rouini, MR., Ardakani, Y.H., Foroumadi, A. et al. Sensitive Quantification of Carboxylic Acid Metabolite of Clopidogrel in Human Plasma by LC with UV Detection. Chroma 70, 953–956 (2009). https://doi.org/10.1365/s10337-009-1245-2

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  • DOI: https://doi.org/10.1365/s10337-009-1245-2

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