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Evaluation of Two Techniques for Extraction of Cortisol from Human Saliva

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Abstract

The aim of the study was to compare two kinds of extraction of cortisol from human saliva. The first was made with dichloromethane and the second by solid-phase extraction. Saliva was sampled in the evening into plastic tubes then cortisol was extracted and analyzed by LC with detection at 240 nm. The limit of detection was 1 ng mL−1 for extraction with dichloromethane and 3 ng mL−1 for SPE extraction. Both methods of extraction were applied to the analysis of saliva samples without any interference peaks.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland

    Ewelina Dziurkowska & Marek Wesolowski

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  1. Ewelina Dziurkowska
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  2. Marek Wesolowski
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Correspondence to Marek Wesolowski.

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Dziurkowska, E., Wesolowski, M. Evaluation of Two Techniques for Extraction of Cortisol from Human Saliva. Chroma 70, 769–774 (2009). https://doi.org/10.1365/s10337-009-1239-0

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  • DOI: https://doi.org/10.1365/s10337-009-1239-0

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