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Sensitive Analysis of Malondialdehyde in Human Urine by Derivatization with Pentafluorophenylhydrazine then Headspace GC–MS

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Abstract

A sensitive method based on derivatization with pentafluorophenylhydrazine then headspace gas chromatography–mass spectrometry has been used for analysis of malondialdehyde in human urine. Preparation of urine sample by one-step derivatization/evaporation was performed by reaction of malondialdehyde with pentafluorophenylhydrazine in a headspace vial for 10 min; the derivatives were then injected in GC–MS analysis. The reaction was performed at pH 3, and total analysis time was 35 min. The method detection limit was 0.04 μg L−1. For MDA concentrations of 2.0 and 10.0 μg L−1 the relative standard deviation was less then 5%. The concentration of MDA in urine was measured to be 0.199 ± 0.252 μmol g−1 creatinine (0.022 ± 0.028 μmol mmol−1 creatinine).

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Authors and Affiliations

  1. Department of Environmental Education, Kongju National University, Kongju, 314-701, Republic of Korea

    Ho-Sang Shin

  2. Department of Environmental Science, Graduate School, Kongju National University, Kongju, 314-701, Republic of Korea

    Dong-Gyun Jung

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  1. Ho-Sang Shin
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  2. Dong-Gyun Jung
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Correspondence to Ho-Sang Shin.

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Shin, HS., Jung, DG. Sensitive Analysis of Malondialdehyde in Human Urine by Derivatization with Pentafluorophenylhydrazine then Headspace GC–MS. Chroma 70, 899–903 (2009). https://doi.org/10.1365/s10337-009-1235-4

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  • DOI: https://doi.org/10.1365/s10337-009-1235-4

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