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LC Characterization of the Major Constituents in Zhi-Zi-Hou-Pu Decoction Using Various Detection Approaches

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Abstract

This study describes the development of various detection approaches to identify the major constituents in a classic formula Zhi-Zi-Hou-Pu decoction without use of reference standards. Three techniques such as LC with ultraviolet-visible detection, LC with fluorescence detection, and LC with photo-diode array detection-electrospray ionization-tandem mass spectrometry produced a considerable effect. The chromatographic and spectral information obtained as well as the recorded literature of the herbs were well utilized to identify the multiple ingredients of the formula to a great extent. The proposed techniques were then successfully applied to identify twelve compounds such as genipin-1-β-gentiobioside, geniposide, eriocitrin, neoeriocitrin, isonaringin, naringin, hesperidin, neohesperidin, naringenin, hesperetin, honokiol, and magnolol in Zhi-Zi-Hou-Pu decoction.

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Acknowledgements

This study was sponsored by Qing Lan Project of Jiangsu Province and the Innovative Foundation of Simcere Pharmaceutical Company for Postgraduates in China Pharmaceutical University.

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis, China Pharmaceutical University, 210009, Nanjing, People’s Republic of China

    Lan Zhu, Fang Feng, Xiaomin Ye, Zhe Wang & Lulu Zhang

  2. Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Ministry of Education, 210009, Nanjing, People’s Republic of China

    Fang Feng

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  1. Lan Zhu
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  2. Fang Feng
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  3. Xiaomin Ye
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  4. Zhe Wang
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  5. Lulu Zhang
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Correspondence to Fang Feng.

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Zhu, L., Feng, F., Ye, X. et al. LC Characterization of the Major Constituents in Zhi-Zi-Hou-Pu Decoction Using Various Detection Approaches. Chroma 70, 975–980 (2009). https://doi.org/10.1365/s10337-009-1234-5

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  • DOI: https://doi.org/10.1365/s10337-009-1234-5

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