Abstract
A sensitive and selective liquid chromatography–tandem mass spectrometry method for the determination of memantine was developed and validated over the linearity range 0.1–25 ng mL−1 with 0.5 mL of plasma using procainamide as the internal standard. This analysis was carried out on a Cosmosil 5C18-MS column and the mobile phase was composed of methanol: 0.5% formic acid (50:50, v/v). Detection was performed on a triple–quadrupole tandem mass spectrometer using positive ion mode electrospray ionization and quantification was performed by multiple reaction monitoring mode. The MS–MS ion transitions monitored were m/z 180 → 107 and 236 → 163 for memantine and procainamide, respectively. The between- and within-day precision was less than 10.9% and accuracy was less than 2.5%. The lower limit of quantification (LLOQ) was 0.1 ng mL−1. The method proved to be accurate and specific, and was applied to the pharmacokinetic study of memantine in healthy Chinese volunteers.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Parsons CG, Danysz W, Quack G (1999) Neuropharmacology 38:735–767. doi:10.1016/S0028-3908(99)00019-2
Greenamyre JT, Maragos WF, Albin RL, Penney JB, Young AB (1988) Prog Neuropsychopharmacol Biol Psychiatry 12:421–430. doi:10.1016/0278-5846(88)90102-9
Reisberg B, Doody R, Stoffler A, Schmitt F, Ferris S, Mobius HJ (2003) N Engl J Med 348:1333–1341. doi:10.1056/NEJMoa013128
Ditzler K (1991) Arzneim-Forsch 41:773–780
Kornhuber J, Weller M, Schoppmeyer K, Riederer P (1994) J Neural Transm Suppl 43:91–104
Sonkusare SK, Kaul CL, Ramarao P (2005) Pharmacol Res 51:1–17
Periclou A, Ventura D, Rao N, Abramowitz W (2006) Clin Pharmacol Ther 79:134–143. doi:10.1016/j.clpt.2005.10.005
Collins ED, Ward AS, McDowell DM, Foltin RW, Fischman MW (1998) Behav Pharmacol 9:587–598. doi:10.1097/00008877-199811000-00014
Kornhuber J, Quack G (1995) Neurosci Lett 195:137–139. doi:10.1016/0304-3940(95)11785-U
Wesemann W, Schollmeyer JD, Sturm G (1982) Arzneim-Forsch 32:1243–1245
Freudenthaler S, Meineke I, Schreeb KH, Boakye E, Gunder-Remy U, Gleiter CH (1998) Br J Clin Pharmacol 46:541–546. doi:10.1046/j.1365-2125.1998.00819.x
Leis HJ, Fauler G, Windischhofer W (2002) J Mass Spectrom 37:477–480. doi:10.1002/jms.303
Suckow RF, Zhang MF, Collins ED, Fischman MW, Cooper TB (1999) J Chromatogr B Biomed Sci Appl 729:217–224. doi:10.1016/S0378-4347(99)00157-7
Suckow RF (2001) J Chromatogr B Biomed Sci Appl 764:313–325. doi:10.1016/S0378-4347(01)00318-8
Zhou FX, Krull IS, Feibush B (1993) J Chromatogr A 619:93–101. doi:10.1016/0378-4347(93)80450-I
Reichova N, Pazourek J, Polaskova P, Havel J (2002) Electrophoresis 23:259–262. doi:10.1002/1522-2683(200202)23:2<259::AID-ELPS259>3.0.CO;2-U
Koeberle MJ, Hughes PM, Wilson CG, Skellern GG (2003) J Chromatogr B Analyt Technol Biomed Life Sci 787:313–322. doi:10.1016/S1570-0232(02)00957-1
Liu MY, Meng SN, Wu HZ, Wang S, Wei MJ (2008) J Clin Ther 30:641–653. doi:10.1016/j.clinthera.2008.04.005
Almeida AA, Campos DR, Bernascone G, Calafatti S, Barros FAP, Eberlin MN, Meurer EC, Paris EG, Pedrazzoli J (2007) J Chromatogr B Analyt Technol Biomed Life Sci 848:311–316. doi:10.1016/j.jchromb.2006.10.045
Food and Drug Administration Center for Drug Evaluation and Research (CDER) Guidance for Industry, Bioanalytical Method Validation (2001) US Department of Health and Human Services
Matuszewski BK, Constanzer ML, Chavez-Eng CM (2003) Anal Chem 75:3019–3030. doi:10.1021/ac020361s
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Pan, RN., Chian, TY., Kuo, B.PC. et al. Determination of Memantine in Human Plasma by LC–MS–MS: Application to a Pharmacokinetic Study. Chroma 70, 783–788 (2009). https://doi.org/10.1365/s10337-009-1231-8
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-009-1231-8