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Determination of Atovaquone in Human Plasma by LC-MS-MS and Its Application to a Bioequivalence Study

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Abstract

A sensitive and selective method for the determination of atovaquone in human plasma was developed and validated. The procedure employed the use of an internal standard (chlorothalidone) and a solvent extraction step. Detection was by electrospray ionization tandem mass spectrometry with multiple reaction monitoring. The method showed a linear range from 50 to 2,000 ng mL−1. The extraction recovery was determined to be 84.91 ± 6.42% (SD), the intra- and inter-day assay accuracy (relative error) was within 7.57% and precision (RSD) was below 6.06%. The method was successfully employed to analyze plasma samples and evaluate the pharmacokinetics of atovaquone in human volunteers.

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Authors and Affiliations

  1. Department of Chemistry, Mithibai College of Arts, Chaun Institute of Science and Amrutben Jivanlal College of Commerce and Economics, Vile Parle (W), Mumbai, 400056, India

    Satish Gangaram Pingale & Kiran V. Mangaonkar

  2. Drug Monitoring Research Institute, Rabale, Navi Mumbai, 400709, India

    Satish Gangaram Pingale, Ketan K. Nerurkar, Ajit M. Padgaonkar & Uttam D. Pawar

Authors
  1. Satish Gangaram Pingale
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  2. Ketan K. Nerurkar
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  3. Ajit M. Padgaonkar
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  4. Uttam D. Pawar
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  5. Kiran V. Mangaonkar
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Correspondence to Satish Gangaram Pingale.

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Pingale, S.G., Nerurkar, K.K., Padgaonkar, A.M. et al. Determination of Atovaquone in Human Plasma by LC-MS-MS and Its Application to a Bioequivalence Study. Chroma 70, 947–951 (2009). https://doi.org/10.1365/s10337-009-1220-y

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  • DOI: https://doi.org/10.1365/s10337-009-1220-y

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