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Establishment of a Fingerprint of Raspberries by LC

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Abstract

To monitor the quality of raspberries, a simple, practicable, and feasible method, high-performance liquid chromatography coupled with photodiode-array detection, has been developed both for fingerprinting analysis and for quantitative analysis of hyperoside and tiliroside. Compounds were separated on a 4.6 mm × 250 mm, 5-μm particle, C18 column with a mobile phase gradient prepared from acetonitrile and 0.5% (v/v) aqueous glacial acetic acid. The mobile phase flow rate was 1.0 mL min−1, the temperature 30 °C, the injection volume 10 μL, and the detection wavelength 340 nm. Under the optimum conditions the two compounds could be well separated with good linear relationships between response and amount in the range 0.024–2.380 μg for hyperoside and 0.026–2.570 μg for tiliroside. Recovery of hyperoside and tiliroside was 99.3 and 106.0%, respectively. The standardized chromatographic fingerprint and the similarity of samples were calculated by use of software. Principal-components analysis was used to differentiate and classify 14 samples on the basis of the area of seven common peaks. Combination of chemical fingerprinting and the quantitative method with principal-component analysis may provide a firm basis for further research on quality control of raspberries.

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Acknowledgments

This work was supported by the Shanghai Leading Academic Discipline Project (B505) and by the National Special Fund for State Key Laboratory of Bioreactor Engineering, grant no. 2060204.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Box 283#, 130 Meilong Road, 200237, Shanghai, People’s Republic of China

    Juan Chen, Yan-Hua Lu & Dong-Zhi Wei

  2. Speciry Faculty, Shanghai Institute of Technology, 200235, Shanghai, People’s Republic of China

    Xiao-Li Zhou

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  1. Juan Chen
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  2. Yan-Hua Lu
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  3. Dong-Zhi Wei
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  4. Xiao-Li Zhou
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Correspondence to Yan-Hua Lu.

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Chen, J., Lu, YH., Wei, DZ. et al. Establishment of a Fingerprint of Raspberries by LC. Chroma 70, 981–985 (2009). https://doi.org/10.1365/s10337-009-1217-6

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