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The Use of Contactless Conductivity for the On-Column Characterisation and Visualisation of Packing Homogeneity and Band Broadening in Capillary LC

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Abstract

Capacitively coupled contactless conductivity is presented as a simple non-invasive tool for the visualisation and evaluation of the axial homogeneity of packing density in capillary LC columns. Irregularities in frit structure and gross column voids were readily identified, the latter of which was confirmed with digital photography. Relative homogeneity of packing density was compared for two packed columns by measuring the variation in conductive response per unit length and varied from 1.1 to 4.2%. The on-column scanning methodology was applied to real-time visualisation of in-situ buffering effects arising from a packed poly-iminodiacetic acid bonded resin. Finally, the scanning detection technique was applied to the pre-elution visualisation of on-column band broadening.

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References

  1. Colón LA, Maloney TD, Fermier AM (2000) J Chromatogr A 887:43–53. doi:10.1016/s0021-9673(99)01328-x

    Article  Google Scholar 

  2. Boughtflower RJ, Underwood T, Paterson CJ (1995) Chromatographia 40:329–335. doi:10.1007/BF02290365

    Article  CAS  Google Scholar 

  3. Smith NW, Evans MB (1994) Chromatographia 38:649–657. doi:10.1007/BF02277170

    Article  CAS  Google Scholar 

  4. Boughtflower RJ, Underwood T, Maddin J (1995) Chromatographia 41:398–402. doi:10.1007/BF02318612

    CAS  Google Scholar 

  5. Robson MM, Roulin S, Shariff SM, Raynor MW, Bartle KD, Clifford AA, Myers P, Euerby MR, Johnson CM (1996) Chromatographia 43:313–321. doi:10.1007/BF02271002

    Article  CAS  Google Scholar 

  6. Maloney TD, Colón LA (1999) Electrophoresis 20:2360–2365. doi:10.1002/(SICI)1522-2683(19990801)20:12<2360:AID-ELPS2360>3.0.CO;2-N

    Article  CAS  Google Scholar 

  7. Stol R, Mazereeuw M, Tjaden UR, van der Greef J (2000) J Chromatogr A 873:293–298. doi:10.1016/s0021-9673(99)01305-9

    Article  CAS  Google Scholar 

  8. van den Bosch SE, Heemstra S, Kraak JC, Poppe H (1996) J Chromatogr A 755:165–177. doi:10.1016/s0021-9673(96)00612-7

    Article  Google Scholar 

  9. Kubáň P, Hauser PC (2004) Electroanalysis 16:2009–2021. doi:10.1002/elan.200403125

    Article  Google Scholar 

  10. Kubáň P, Hauser PC (2008) Anal Chim Acta 607:15–29. doi:10.1016/j.aca.2007.11.045

    Article  Google Scholar 

  11. Kubáň P, Hauser PC (2004) Electrophoresis 25:3387–3397. doi:10.1002/elps.200406059

    Article  Google Scholar 

  12. Gillespie E, Connolly D, Macka M, Paull B (2006) Analyst (Lond) 131:886–888. doi:10.1039/b606942h

    Article  CAS  Google Scholar 

  13. Gillespie E, Connolly D, Macka M, Nesterenko PN, Paull B (2007) Analyst (Lond) 132:1238–1245. doi:10.1039/b711350a

    Article  CAS  Google Scholar 

  14. Connolly D, O’Shea V, Clarke P, O’Connor B, Paull B (2007) J Sep Sci 30:3060–3068. doi:10.1002/jssc.200700365

    Article  CAS  Google Scholar 

  15. Wong V, Shalliker RA, Guiochon G (2004) Anal Chem 76:2601–2608. doi:10.1021/ac030391a

    Article  CAS  Google Scholar 

  16. Tyrrell E, Hilder EF, Shalliker RA, Dicinoski GW, Shellie RA, Breadmore MC, Pohl CA, Haddad PR (2008) J Chromatogr A 1208:95–100. doi:10.1016/j.chroma.2008.08.056

    Article  CAS  Google Scholar 

  17. Gillespie E, Connolly D, Macka M, Hauser P, Paull B (2008) Analyst 133:1104–1110. doi:10.1039/b803038c

    Article  CAS  Google Scholar 

  18. Dionex Corporation, ProPac IMAC-10 Column Manual, Document Number: 065090, January 2007

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Acknowledgments

The authors would like to acknowledge Science Foundation Ireland (Grant Number 08/SRC/B1412) for research funding under the Strategic Research Cluster programme.

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Authors and Affiliations

  1. Irish Separation Science Cluster, National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin, Ireland

    Damian Connolly, Leon P. Barron, Eoin Gillespie & Brett Paull

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  1. Damian Connolly
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  2. Leon P. Barron
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  3. Eoin Gillespie
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  4. Brett Paull
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Correspondence to Brett Paull.

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Connolly, D., Barron, L.P., Gillespie, E. et al. The Use of Contactless Conductivity for the On-Column Characterisation and Visualisation of Packing Homogeneity and Band Broadening in Capillary LC. Chroma 70, 915–920 (2009). https://doi.org/10.1365/s10337-009-1214-9

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  • DOI: https://doi.org/10.1365/s10337-009-1214-9

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