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LC Determination of Lidocaine and Prilocaine Containing Potential Risky Impurities and Application to Pharmaceuticals

Chromatographia volume 70, pages 563–568 (2009)Cite this article

An Erratum to this article was published on 07 August 2009

Abstract

A liquid chromatographic method for the determination of lidocaine (LID), prilocaine (PRL) and their impurities 2,6-dimethylaniline (DMA) and o-toluidine (TOL) has been developed. The analysis was performed on a reversed phase C18 Hypersil BDS column at ambient temperature. A mobile phase consisting of Briton-Robinson buffer, pH 7—methanol—acetonitrile (40: 45: 15 v/v/v) was used at a flow rate of 1.2 mL min−1. Detection was achieved at 225 nm using benzophenone as internal standard over the concentration range 1.25–80 μg mL−1 for all analytes. The relative standard deviations RSD (n = 7) for the assay were less than 0.95%. Limit of detection values were found to be 0.346, 0.423, 0.112 and 0.241 μg mL−1 for LID, PRL, DMA and TOL, respectively. The intraday and the inter-days RSD % indicated the precision of the procedure. The method proved to be suitable for the quality control of LID and PRL in pharmaceuticals.

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Authors and Affiliations

  1. Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini, Cairo, 11562, Egypt

    Mohammad Abdul-Azim Mohammad

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  1. Mohammad Abdul-Azim Mohammad
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Correspondence to Mohammad Abdul-Azim Mohammad.

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An erratum to this article can be found at http://dx.doi.org/10.1365/s10337-009-1274-x

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Mohammad, M.AA. LC Determination of Lidocaine and Prilocaine Containing Potential Risky Impurities and Application to Pharmaceuticals. Chroma 70, 563–568 (2009). https://doi.org/10.1365/s10337-009-1173-1

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  • DOI: https://doi.org/10.1365/s10337-009-1173-1

Keywords

  • Column liquid chromatography
  • Pharmaceutical study
  • Lidocaine
  • Prilocaine and impurities