Skip to main content

Determination of Histamine in Rat Plasma and Tissue Extracts by Intramolecular Excimer-Forming Derivatization and LC with Fluorescence Detection


A highly sensitive and selective method for determination of histamine in rat plasma and tissue extracts by liquid chromatography with fluorescence detection is described. The method is based on precolumn derivatization of amino groups of histamine with two molecules of 4-(1-pyrene)butyric acid N-hydroxysuccinimide ester that allows generation of intramolecular excimer fluorescence. The detection limit for histamine was 0.183 nM. This sensitivity allowed determination of histamine in 10 μL of rat plasma or in the extracts from less than 1 mg of tissue.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4


  1. Beaven MA, Jacobsen S, Horáková Z (1972) Clin Chim Acta 37:91–103. doi:10.1016/0009-8981(72)90419-6

    Article  CAS  Google Scholar 

  2. Snyder SH, Baldessarini RJ, Axelrod J (1966) J Pharmacol Exp Ther 153:544–549

    CAS  Google Scholar 

  3. Kobayashi Y, Maudsley DV (1972) Anal Biochem 46:85–90. doi:10.1016/0003-2697(72)90397-1

    Article  CAS  Google Scholar 

  4. Keyzer JJ, Wolthers BG, Muskiet FAJ, Breukelman H, Kauffman HF, de Vries K (1984) Anal Biochem 139:474–481. doi:10.1016/0003-2697(84)90037-X

    Article  CAS  Google Scholar 

  5. Pittertschatscher K, Hochreiter R, Thalhamer J, Hammerl P (2002) Anal Biochem 308:300–306. doi:10.1016/S0003-2697(02)00260-9

    Article  CAS  Google Scholar 

  6. Davis TP, Gehrke CW, Gehrke CW Jr, Cunningham TD, Kuo KC, Gerhardt KO, Johnson HD, Williams CH (1978) Clin Chem 24:1317–1324

    CAS  Google Scholar 

  7. Tsuruta Y, Kohashi K, Ohkura Y (1981) J Chromatogr 224:105–110

    Article  CAS  Google Scholar 

  8. Yamatodani A, Fukuda H, Wada H, Iwaeda T, Watanabe T (1985) J Chromatogr 344:115–123

    Article  CAS  Google Scholar 

  9. Mine K, Jacobson KA, Kirk KL, Kitajima Y, Linnoila M (1986) Anal Biochem 152:127–135. doi:10.1016/0003-2697(86)90130-2

    Article  CAS  Google Scholar 

  10. Oguri S, Yoneya Y (2002) J Chromatogr B Analyt Technol Biomed Life Sci 781:165–179. doi:10.1016/S1570-0232(02)00534-2

    Article  CAS  Google Scholar 

  11. Skofitsch G, Saria A, Holzer P, Lembeck F (1981) J Chromatogr 226:53–59. doi:10.1016/S0378-4347(00)84205-X

    Article  CAS  Google Scholar 

  12. Yoshitake T, Ichinose F, Yoshida H, Todoroki K, Kehr J, Inoue O, Nohta H, Yamaguchi M (2003) Biomed Chromatogr 17:509–516. doi:10.1002/bmc.264

    Article  CAS  Google Scholar 

  13. Johnson KB, Charya RV, Wiesmann WP, Pearce FJ (1995) Shock 3:343–349. doi:10.1097/00024382-199505000-00005

    Article  CAS  Google Scholar 

  14. Arakawa Y, Tachibana S (1986) Anal Biochem 158:20–27. doi:10.1016/0003-2697(86)90582-8

    Article  CAS  Google Scholar 

  15. Florjanc TI, Erjavec F (1987) Agents Actions 20:194–197. doi:10.1007/BF02074667

    Article  CAS  Google Scholar 

  16. Izumi H, Ho S, Michelakis AM, Aoki T (1985) Eur J Pharmacol 109:249–256. doi:10.1016/0014-2999(85)90426-1

    Article  CAS  Google Scholar 

  17. Wang JP, Raung SL, Lin CN, Teng CM (1994) Eur J Pharmacol 251:35–42. doi:10.1016/0014-2999(94)90440-5

    Article  CAS  Google Scholar 

  18. Shin TY, Kim SH, Kim SH, Kim YK, Park HJ, Chae BS, Jung HJ, Kim HM (2000) Immunopharmacol Immunotoxicol 22:489–500. doi:10.3109/08923970009026007

    Article  CAS  Google Scholar 

Download references


This work was supported in part by a Grant-in-Aid for Scientific Research (C) (No. 14572043) from the Ministry of Education, Science, Sports and Culture of Japan, and Advanced Materials Institute, Fukuoka University.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Masatoshi Yamaguchi.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ichinose, F., Yoshitake, T., Yoshida, H. et al. Determination of Histamine in Rat Plasma and Tissue Extracts by Intramolecular Excimer-Forming Derivatization and LC with Fluorescence Detection. Chroma 70, 575–580 (2009).

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:


  • Column liquid chromatography
  • Fluorescence derivatization
  • Intramolecular excimer
  • Histamine in rat plasma and tissue
  • 4-(1-Pyrene)butyric acid N-hydroxysuccinimide ester