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Polypyrrole-Coated and Polysulfate-Modified CE Capillaries

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Abstract

Fused-silica capillaries covalently coated with polypyrrole offer some interesting properties for capillary electrophoresis. The polymeric, surface-bonded coating is chemically stable and reduces the adsorptive properties of the silica surface. At the same time, the weak positive surface charges offer the possibility of creating a strongly anionic surface when poly-sulfates are used as counter-ions. This affords an electroosmotic flow which is largely independent of pH. With sodium dodecyl sulfate in the background electrolyte, this effect is further enhanced. The potential of the approach is demonstrated with the separation of herbicides by micellar electrokinetic chromatography. Repeatability of electroosmotic mobility of less than 1% RSD is achieved.

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Acknowledgment

The authors are grateful to the Ministry of Education of the Czech Republic (grant No. 6198959216) and to the Grant Agency of the Czech Republic (grant No 203/07/P233).

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Authors and Affiliations

  1. Environmental Chemistry and Ecotoxicology, University of Bayreuth, 95440, Bayreuth, Germany

    Radim Knob, Silke Gerstmann & Hartmut Frank

  2. Department of Analytical Chemistry, Faculty of Science, Palacký University, Třída Svobody 8, 771 46, Olomouc, Czech Republic

    Radim Knob

  3. Department of Analytical Chemistry, Faculty of Science, Charles University, Hlavova 2030/8, 128 43, Prague, Czech Republic

    Radomír Čabala

Authors
  1. Radim Knob
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  2. Silke Gerstmann
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  3. Radomír Čabala
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  4. Hartmut Frank
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Corresponding author

Correspondence to Hartmut Frank.

Additional information

Dedicated to Gerhard Schomburg on the occasion of his 80th birthday.

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Knob, R., Gerstmann, S., Čabala, R. et al. Polypyrrole-Coated and Polysulfate-Modified CE Capillaries. Chroma 69, 1431–1435 (2009). https://doi.org/10.1365/s10337-009-1098-8

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  • DOI: https://doi.org/10.1365/s10337-009-1098-8

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