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LC Separation of Fatty Acid Ceramides Using a Two Column System

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Abstract

Type II ceramides were separated according to the length of their fatty acid alkyl chain using two column system. The packing of the first column was non-polar adsorbent prepared by coating of macroporous spherical silica with cationized poly(vinyl alcohol) of low substitution degree. Commercial normal phase column Lichrosorb Si 60 was used as a second column in this system. Elution was performed in an isocratic mode using methanol:chloroform 50:50 (v/v) as an eluent.

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References

  1. Gaudin K, Chaminade P, Baillet A (2002) J Chromatogr A 973:69–83. doi:10.1016/S0021-9673(02)01120-2

    Article  CAS  Google Scholar 

  2. Raith K, Neubert RHH (2000) Anal Chim Acta 403:295–303. doi:10.1016/S0003-2670(99)00661-3

    Article  CAS  Google Scholar 

  3. Molander P, Holm A, Lundanes E, Greibrokk T, Ommudsen E (2000) J High Resol Chromatogr 23:653–655

    Article  CAS  Google Scholar 

  4. Raith K, Farwanah H, Wartewig S, Neubert RHH (2004) Eur J Lipid Technol 106:561–571. doi:10.1002/ejlt.200400982

    Article  CAS  Google Scholar 

  5. Farwanah H, Nuhn P, Neubert R, Raith K (2003) Anal Chim Acta 492:233–239. doi:10.1016/S0003-2670(03)00303-9

    Article  CAS  Google Scholar 

  6. Christie WW, Urwin RA (1995) J High Resol Chromatogr 18:97–100

    Article  CAS  Google Scholar 

  7. Nordback J, Lundberg E, Christie WW (1998) Mar Chem 60:165–175. doi:10.1016/S0304-4203(97)00109-6

    Article  CAS  Google Scholar 

  8. McNabb TJ, Cremesti AE, Brown PR, Fischl AS (1999) Anal Biochem 276:242–250. doi:10.1006/abio.1999.4354

    Article  CAS  Google Scholar 

  9. Norlen L, Nicander I, Lundsjo A, Cronholm T, Forslind B (1998) Arch Dermatol Res 290:508–516. doi:10.1007/s004030050344

    Article  CAS  Google Scholar 

  10. Deschamps FS, Chaminade P, Ferrier D, Baillet A (2001) J Chromatogr A 928:127–137. doi:10.1016/S0021-9673(01)01137-2

    Article  CAS  Google Scholar 

  11. Quinton L, Gaudin K, Baillet A, Chaminade P (2006) J Sep Sci 29:390–398. doi:10.1002/jssc.200500362

    Article  CAS  Google Scholar 

  12. Lesellier E, Gaudin K, Chaminade P, Tchapla A, Baillet A (2003) J Chromatogr A 1016:111–127. doi:10.1016/S0021-9673(03)01323-2

    Article  CAS  Google Scholar 

  13. Shabbits JA, Mayer LD (2003) Biochim Biophys Acta 1612:98–106. doi:10.1016/S0005-2736(03)00108-1

    Article  CAS  Google Scholar 

  14. Kent KD, Clubbs EA, Harper WJ, Bomser JA (2008) Lipids 43:143–149. doi:10.1007/s11745-007-3148-z

    Article  CAS  Google Scholar 

Download references

Acknowledgement

The study was supported by the Lithuanian State Science and Studies Foundation (contracts No V-23/2008 and T-29/08).

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Authors and Affiliations

  1. Kaunas University of Technology, Radvilenu pl. 19, 50254, Kaunas, Lithuania

    Monika Budvytiene & Jolanta Liesiene

  2. Institute of Biology, Kharkov Karazin National University, 4 Svobody pl., 61077, Kharkov, Ukraina

    Nataliya Babenko

Authors
  1. Monika Budvytiene
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  2. Nataliya Babenko
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  3. Jolanta Liesiene
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Correspondence to Jolanta Liesiene.

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Budvytiene, M., Babenko, N. & Liesiene, J. LC Separation of Fatty Acid Ceramides Using a Two Column System. Chroma 69, 1473–1475 (2009). https://doi.org/10.1365/s10337-009-1093-0

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  • DOI: https://doi.org/10.1365/s10337-009-1093-0

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