Abstract
The reliability of evaluation of compound purity depends on the analytical method selected. A suitable analytical method, including the modes of separation and detection, can provide reliable information about compound purity. Effective separation is the key to achieving precise results. In this work we developed a novel method, based on an orthogonal system, RP-LC-hydrophilic interaction liquid chromatography, for evaluating the purity of compounds isolated by preparative reversed-phase liquid chromatography. Because of its good orthogonality, good separation and more accurate assessment of compound purity could be achieved.
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Acknowledgment
This work was supported by the “Science and Technology Plan of Liaoning Province (2006226002)”, the “National Natural Science Funds (20775079)”, and “Programs Funded by Ministry of Science and Technology of China During the 11th Five-Year Plan (2006BAI06A01-06)”.
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Wang, Y., Zhang, F., Xue, X. et al. Use of an Orthogonal System, RP-LC–HILIC, for Evaluation of Purity. Chroma 69, 1379–1384 (2009). https://doi.org/10.1365/s10337-009-1077-0
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DOI: https://doi.org/10.1365/s10337-009-1077-0