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Investigation of the Interaction Between Lidocaine and the Components of Hyaluronic Acid Using Frontal Analysis Continuous Capillary Electrophoresis

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Abstract

The frontal analysis continuous capillary electrophoresis (FACCE) technique was used for the characterziation of the interaction between lidocaine-HCl (Lido) and components of Na-hyaluronic acid (HA). N-Acetylglucosamine (GlcNAc) and glucuronic acid (GluA) were the components of Na-hyaluronic acid. For the investigations fused silica capillaries were used. The FACCE method was compared to affinity capillary electrophoresis (ACE). The association constants between lidocaine-HCl and the components of Na-hyaluronic acid were determined using FACCE. It was observed that only an interaction between Lido and GluA exhibited. The association constant (K lido-GluA) between Lido and GluA was 26 ± 0.3 L mol−1.

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Authors and Affiliations

  1. Institute of Applied Dermatopharmacy, Martin-Luther-University Halle-Wittenberg, Wolfgang-Langenbeck Str. 4, 06120, Halle, Germany

    Yahya Mrestani

  2. Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Wolfgang-Langenbeck-Str. 4, 06120, Halle, Germany

    Manuela Hammitzch & Reinhard H. H. Neubert

Authors
  1. Yahya Mrestani
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  2. Manuela Hammitzch
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  3. Reinhard H. H. Neubert
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Correspondence to Yahya Mrestani.

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Mrestani, Y., Hammitzch, M. & Neubert, R.H.H. Investigation of the Interaction Between Lidocaine and the Components of Hyaluronic Acid Using Frontal Analysis Continuous Capillary Electrophoresis. Chroma 69, 1321–1324 (2009). https://doi.org/10.1365/s10337-009-1075-2

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  • DOI: https://doi.org/10.1365/s10337-009-1075-2

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