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LC–MS Simultaneous Determination of Itopride Hydrochloride and Domperidone in Human Plasma

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Abstract

A rapid, simple, sensitive and specific liquid chromatography–tandem mass spectrometry method was developed and validated for simultaneous quantification of itopride hydrochloride and domperidone in human plasma. Both drugs were extracted by liquid–liquid extraction with ethyl acetate and saturated borax solution. The chromatographic separation was performed on a reversed-phase C18 column with a mobile phase of water–methanol (2:98, v/v) containing 0.5% formic acid. The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The assay exhibited linearity over the concentration range of 3.33–500 ng mL−1 for itopride hydrochloride and 3.33–100 ng mL−1 for domperidone in human plasma. The precursor to product ion transitions of m/z 359.1–72.3 and 426.0–147.2 were used to measure itopride hydrochloride and domperidone respectively. The method was found suitable for the analysis of plasma samples collected during phase 1 pharmacokinetics study of itopride HCl 50 mg and domperidone 20 mg in 12 healthy volunteers after single oral doses of the combination drug.

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References

  1. Sretenovic A, Perisic V, Simic A, Zivanovic D, Vujovic D, Kostic M, Pesko P, Krstic Z (2008) Acta Chir Iugosl 55 55(1):47–53. doi:10.2298/ACI0801047S

    Article  CAS  Google Scholar 

  2. Boparai V, Rajagopalan J, Triadafilopoulos G (2008) Drugs 68(7):925–947. doi:10.2165/00003495-200868070-00004

    Article  CAS  Google Scholar 

  3. Katagiri F, Shiga T, Inoue S, Sato Y, Itoh H, Takeyama M (2006) Pharmacology 77:115–121. doi:10.1159/000093485

    Article  CAS  Google Scholar 

  4. Mushiroda T, Douya R, Takahara E, Nagata O (2000) Drug Metab Dispos 28:1231–1237

    CAS  Google Scholar 

  5. Iwanaga Y, Miyashita N, Mizutani F, Morikawa K, Kato H, Ito Y, Itoh Z (1991) Jpn J Pharmacol 56:261–269. doi:10.1254/jjp.56.261

    Article  CAS  Google Scholar 

  6. Iwanaga Y, Miyashita N, Morikawa K, Mizumoto A, Kondo Y, Itoh Z (1990) Gastroenterology 99:401–408

    CAS  Google Scholar 

  7. Iwanaga Y, Suzuki N, Kato K, Kimura T, Morikawa K, Kato H, Ito Y, Gomi Y (1993) Jpn J Pharmacol 62:395–401. doi:10.1254/jjp.62.395

    Article  CAS  Google Scholar 

  8. Lee HW, Seo JH, Choi SK, Lee KT (2007) Anal Chim Acta 583:118–123. doi:10.1016/j.aca.2006.09.061

    Article  CAS  Google Scholar 

  9. Wu MS, Gao L, Cai XH, Wang GJ (2002) Acta Pharmacol Sin 23(3):285–288

    CAS  Google Scholar 

  10. Kobylińska M, Kobylińska K (2000) J Chromatogr B Analyt Technol Biomed Life Sci 744(1):207–212. doi:10.1016/S0378-4347(00)00245-0

    Article  Google Scholar 

  11. Michaud V, Simard C, Turgeon J (2007) J Chromatogr B Analyt Technol Biomed Life Sci 852(1–2):611–616. doi:10.1016/j.jchromb.2007.02.044

    CAS  Google Scholar 

  12. Jung SH, Pham TL, Lim HK, Kim HJ, Kim KH, Kang JS (2000) Arch Pharm Res 23(3):226–229. doi:10.1007/BF02976449

    Article  CAS  Google Scholar 

  13. Rockville MD (2001) Guidance for industry, bioanalytical method validation. US Department of Health and Human Services. Food and Drug Administration. Centre for Drug Evaluation and Research

  14. Gowda KV, Rajan DS, Mandal U, Sam Solomon WD, Senthamil Selvan P, Bose A, Sarkar AK, Chattaraj TK, Pal TK (2007) Asian J. Chem (Kyoto) 19:1293–1300

    CAS  Google Scholar 

  15. Bonfiglio R, King RC, Olah TV, Merkle K (1999) Rapid Commun Mass Spectrom 13:175–1185. doi:10.1002/(SICI)1097-0231(19990630)13:12<1175::AID-RCM639>3.0.CO;2-0

    Article  Google Scholar 

  16. Gowda KV, Rajan DS, Mandal U, Sam Solomon WD, Senthamil Selvan P, Bose A, Sarkar AK, Chattaraj TK, Pal TK (2007) Asian J. Chem (Kyoto) 19:1293–1300

    CAS  Google Scholar 

  17. Annesley TM (2003) Clin Chem 49:1041–1044. doi:10.1373/49.7.1041

    Article  CAS  Google Scholar 

  18. Sun D, Wang H, Wang B, Guo R (2006) J Pharm Biomed Anal 42(3):372–378. doi:10.1016/j.jpba.2006.04.019

    Article  CAS  Google Scholar 

  19. Commission decision of 12 August 2002 implementing Council Directive96/23/EC concerning the performance of analytical methods and the interpretation of the results (2002) Off J Eur Commun 8:L221

    Google Scholar 

  20. Matuszewski BK, Constanzer ML, Chavez-Eng CM (2003) Anal Chem 75:3019–3030. doi:10.1021/ac020361s

    Article  CAS  Google Scholar 

  21. Tan ZR, Oyuang DS, Zhou G, Wang LS, Li Z, Wang D, Zhou HH (2006) J Pharm Biomed Anal 42:207–212. doi:10.1016/j.jpba.2006.02.057

    Article  CAS  Google Scholar 

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Acknowledgments

The authors are thankful to the Department of Science and Technology (DST), Govt. of India, New Delhi, under Pharmaceuticals Research & Development Support Fund (PRDSF) for providing the financial assistance to carry out this research work through their project No. VII–PRDSF/56/05–06–TT.

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Authors and Affiliations

  1. Department of Pharmaceutical Technology, Bioequivalence Study Centre, Jadavpur University, Kolkata, 700 032, India

    Anirbandeep Bose, Uttam Bhaumik, Animesh Ghosh, Bappaditya Chatterjee, Uday Sankar Chakrabarty, Amlan Kanti Sarkar & Tapan Kumar Pal

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  1. Anirbandeep Bose
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  2. Uttam Bhaumik
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  3. Animesh Ghosh
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  4. Bappaditya Chatterjee
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  5. Uday Sankar Chakrabarty
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  6. Amlan Kanti Sarkar
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  7. Tapan Kumar Pal
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Correspondence to Tapan Kumar Pal.

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Bose, A., Bhaumik, U., Ghosh, A. et al. LC–MS Simultaneous Determination of Itopride Hydrochloride and Domperidone in Human Plasma. Chroma 69, 1233–1241 (2009). https://doi.org/10.1365/s10337-009-1032-0

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  • DOI: https://doi.org/10.1365/s10337-009-1032-0

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