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Stability-Indicating LC Method for Assay of Cholecalciferol

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Abstract

This paper discusses the development of a stability-indicating reversed-phase LC method for analysis of cholecalciferol as the bulk drug and in formulations. The mobile phase was acetonitrile–methanol–water 50:50:2 (v/v). The calibration plot for the drug was linear in the range 0.4–10 μg mL−1. The method was accurate and precise with limits of detection and quantitation of 64 and 215 ng, respectively. Mean recovery was 100.71%. The method was used for analysis of cholecalciferol in pharmaceutical formulations in the presence of its degradation products and commonly used excipients.

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Authors and Affiliations

  1. Pharmaceutical Sciences and Technology Division, University Institute of Chemical Technology (Autonomous), University of Mumbai, Matunga, Mumbai, 400019, India

    Namita D. Desai, Pirthipal P. Singh, Purnima D. Amin & Satishkumar P. Jain

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  1. Namita D. Desai
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  2. Pirthipal P. Singh
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  3. Purnima D. Amin
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  4. Satishkumar P. Jain
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Correspondence to Purnima D. Amin.

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Desai, N.D., Singh, P.P., Amin, P.D. et al. Stability-Indicating LC Method for Assay of Cholecalciferol. Chroma 69, 385–388 (2009). https://doi.org/10.1365/s10337-008-0914-x

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  • DOI: https://doi.org/10.1365/s10337-008-0914-x

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