, Volume 69, Issue 3–4, pp 339–344 | Cite as

Analysis of (−)-Shikimic Acid in Chinese Star Anise by GC–MS with Selected Ion Monitoring

  • Hongcheng Liu
  • Qiwan Li
  • Yin Zhang
  • Yanhong Zhou
Full Short Communication


A sensitive, specific, accurate, and reproducible GC–MS method with selected ion monitoring for quantitative determination of (−)-shikimic acid (SA) in Chinese star anise, using benzoic acid as internal standard, has been developed and validated. The homogeneity of the sample was evaluated by one way analysis of variance. The extraction efficiency of ultrasound-assisted and Soxhlet extraction was compared. Results showed that ultrasonic extraction was as efficient as Soxhlet extraction but was more rapid and simpler. Linearity and recovery were good. The method was used for analysis of (−)-SA in Chinese star anise from different areas in Wenshan state, Yunnan province. It was found that Chinese star anise collected from Pingbian and Funing counties contained more (−)-SA than other samples, with two plants from Pingbian county containing >15% (−)-SA.


Gas chromatography-mass spectrometry Selected ion monitoring (−)-Shikimic acid Chinese star anise (Illiciumverum Hook. f.) 



We thank Mo Shunyan and Dong LinLin, now working on natural products at the University of Illinois at Chicago, for their discussion and revision of this paper.


  1. 1.
    Regoes RR, Bonhoeffer S (2006) Science 312:389–391. doi: 10.1126/science.1122947 CrossRefGoogle Scholar
  2. 2.
    Yarnell A (2005) Chem Eng News 83:22Google Scholar
  3. 3.
    Kramer M, Bongaerts J, Bovenberg R, Kremer S, Muller U, Orf S (2003) Metab Eng 5:277–283. doi: 10.1016/j.ymben.2003.09.001 CrossRefGoogle Scholar
  4. 4.
    Wang XQ, Guo YD, Yang CB (2001) Chin J Mater Med 26:447–449 in ChineseGoogle Scholar
  5. 5.
    Bresnahan G, Manthey F, Howatt K, Chakraborty M (2003) J Agric Food Chem 51:4004–4007. doi: 10.1021/jf0301753 CrossRefGoogle Scholar
  6. 6.
    Silva B, Andrade P, Mendes G, Seabra R, Ferreira M (2002) J Agric Food Chem 50:2313–2317. doi: 10.1021/jf011286+ CrossRefGoogle Scholar
  7. 7.
    Dainiak MB, Galaev IY, Mattiasson B (2002) J Chromatogr A 942:123–131. doi: 10.1016/S0021-9673(01)01345-0 CrossRefGoogle Scholar
  8. 8.
    García Romero E, Sánchez Muñoz G, Martín Alvarez PJ, Cabezudo Ibáñez MD (1993) J Chromatogr A 655:111–117. doi: 10.1016/0021-9673(93)87018-H CrossRefGoogle Scholar
  9. 9.
    Tusseau D, Benoit C (1987) J Chromatogr A 395:323–333. doi: 10.1016/S0021-9673(01)94121-4 CrossRefGoogle Scholar
  10. 10.
    Nagels I, Debeuf C, Esmans E (1980) J Chromatogr A 190:411–417. doi: 10.1016/S0021-9673(00)88246-1 CrossRefGoogle Scholar
  11. 11.
    Mardones C, Hitschfeld A, Contreras A, Lepe K, Gutiérrez L, Baer DV (2005) J Chromatogr A 1085:285–292. doi: 10.1016/j.chroma.2005.06.022 CrossRefGoogle Scholar
  12. 12.
    Petersen IL, Andersen KE, Sorensen JC, Sorensen H (2006) J Chromatogr A 1130:253–258. doi: 10.1016/j.chroma.2006.08.011 CrossRefGoogle Scholar
  13. 13.
    Heimler D, Pieroni A (1994) Chromatographia 38:475–478. doi: 10.1007/BF02269839 CrossRefGoogle Scholar
  14. 14.
    Molnár-Perl I, Vasanits A, Horváth K (1998) Chromatographia 48:111–120. doi: 10.1007/BF02467526 CrossRefGoogle Scholar
  15. 15.
    Horváth K, Molnár-Perl I (1998) Chromatographia 48:120–127. doi: 10.1007/BF02467527 CrossRefGoogle Scholar
  16. 16.
    Jaroszyńska J (2003) Anal Bioanal Chem 377:702–708. doi: 10.1007/s00216-003-2155-z CrossRefGoogle Scholar
  17. 17.
    Liu HC, Li QW, Tang LB (2007) J Zhejiang Univ Sci B 9:272–277. doi: 10.1631/jzus.2007.B0272 CrossRefGoogle Scholar
  18. 18.
    Jesús Ibarz M, Ferreira V, Hernández-Orte P, Loscos N, Cacho J (2006) J Chromatogr A 1116:217–229. doi: 10.1016/j.chroma.2006.03.020 CrossRefGoogle Scholar
  19. 19.
    Guedon DJ, Pasquier BP (1994) J Agric Food Chem 42:679–684. doi: 10.1021/jf00039a015 CrossRefGoogle Scholar
  20. 20.
    Loponen J, Ossipov V, Lempa K, Haukioja E, Pihlaja K (1998) Chemosphere 37:1445–1456. doi: 10.1016/S0045-6535(98)00135-0 CrossRefGoogle Scholar

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Hongcheng Liu
    • 1
    • 2
  • Qiwan Li
    • 1
  • Yin Zhang
    • 2
  • Yanhong Zhou
    • 1
  1. 1.Supervision and Testing Center for Farm Product Quality, Ministry of AgricultureQuality Standard and Testing Technology Institute, Yunnan Academy of Agricultural ScienceKunmingPeople’s Republic of China
  2. 2.College of Biosystem Engineering and Food ScienceUniversity of ZhejiangHangzhouPeople’s Republic of China

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