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High Resolution Gel Permeation Chromatography Followed by GC–ECD for the Determination of Pesticide Residues in Soybeans

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Abstract

An efficient and validated approach for the determination of pesticide residues in soybeans using high-resolution gel permeation chromatography in combination with gas chromatography and electron capture detection is described. Gel permeation chromatography was used to remove interfering fatty components of soybeans before gas chromatographic analysis. The limit of quantification for the seven pesticides studied was between 9 and 46 μg kg−1. The method was applied to different soybean varieties and recoveries were determined to be between 93 and 118% with RSD values below 10%.

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References

  1. National Health Surveillance Agency (ANVISA), 19/03/2002, Resolution RE n 475

  2. Lentza-Rizos C, Avramides EJ, Visi E (2001) J Chromatogr A 921:297–304. doi:10.1016/S0021-9673(01)00874-3

    Article  CAS  Google Scholar 

  3. Cairns T, Sherma J (eds) (1992) Multiresidue analysis of fruits and vegetables. In: Emerging strategies for pesticide analysis, CRC Press, Florida, pp. 79, 84–85

  4. Niessner G, Buchberger W, Eckerstorfer R (1999) J Chromatogr A 846:341–348. doi:10.1016/S0021-9673(99)00198-3

    Article  CAS  Google Scholar 

  5. Liu LB, Hashi Y, Qin YP, Zhou HX, Ling JM (2007) J Chromatogr B Analyt Technol Biomed Life Sci 845:61–68. doi:10.1016/j.jchromb.2006.07.032

    Article  CAS  Google Scholar 

  6. Díez C, Traag WA, Zommer P, Marinelo P, Atienza J (2006) J Chromatogr A 1131:11–23. doi:10.1016/j.chroma.2006.07.046

    Article  CAS  Google Scholar 

  7. Moreno JLF, Liébanas FJA, Frenich AG, Vidal JLM (2006) J Chromatogr A 1111:97–105. doi:10.1016/j.chroma.2006.01.108

    Article  CAS  Google Scholar 

  8. Walters SM (1990) Anal Chim Acta 236:77–82. doi:10.1016/S0003-2670(00)83301-2

    Article  CAS  Google Scholar 

  9. Johnson PD, Rimmer DA, Brown RH (1997) J Chromatogr A 765:3–11. doi:10.1016/S0021-9673(96)00950-8

    Article  CAS  Google Scholar 

  10. Pang GF, Fan CL, Chao YZ (1994) J AOAC Int 77:738–747

    CAS  Google Scholar 

  11. Muccio ADi, Generali T, Barbini DA, Barbini DA, Pelosi P, Ausili A, Vergoni F, Girolimetti S (1997) J Chromatogr A 765:61–68

    Article  Google Scholar 

  12. Castro J, Pérez RA, Miguel E, Sánchez C, Tadeo JL (2002) J Chromatogr A 947:119–127. doi:10.1016/S0021-9673(01)01598-9

    Article  CAS  Google Scholar 

  13. Meloan CE (ed) (1996) Chapter 3: extraction, cleanup and concentration. In: Pesticides laboratory training manual. AOAC International, Maryland, pp. 102–106

  14. Rimkus GG, Rummler M, Nausch I (1996) J Chromatogr A 737:9–14. doi:10.1016/0021-9673(96)00020-9

    Article  CAS  Google Scholar 

  15. Guardia-Rubio M, De Córdoba MLF, Cañada MJA, Medina AR (2006) J Chromatogr A 1108:231–239. doi:10.1016/j.chroma.2006.01.006

    Article  CAS  Google Scholar 

  16. Ault JA, Spurgeon TE (1984) J Assoc Off Anal Chem 67:284–289

    CAS  Google Scholar 

  17. Luke M, Froberg JE, Masumoto HT (1975) J AOAC Int 58:1020–1026

    CAS  Google Scholar 

  18. Roos AH, Munsteren Van AJ, Nab FM, Tuinstra LGMTh (1987) Anal Chim Acta 196:95–102. doi:10.1016/S0003-2670(00)83074-3

    Article  CAS  Google Scholar 

  19. Ainie K, Hamirin K, Tan YA (2000) J Oil Palm Res 12:95–101

    CAS  Google Scholar 

  20. Hoff RG, Zoonen P (1999) J Chromatogr A 843:301–322. doi:10.1016/S0021-9673(99)00511-7

    Article  Google Scholar 

  21. Juhler RK (1997) J Chromatogr A 786:145–153. doi:10.1016/S0021-9673(97)00690-0

    Article  CAS  Google Scholar 

  22. Rossi S, Dalpero AP, Ghini S et al (2001) J Chromatogr A 905:223–232. doi:10.1016/S0021-9673(00)00928-6

    Article  CAS  Google Scholar 

  23. Mukherjee I, Gopal M (1996) J Chromatogr A 754:33–44. doi:10.1016/S0021-9673(96)00426-8

    Article  CAS  Google Scholar 

  24. Sannino A, Mambriani P, Bandini M, Bolzoni R (1995) J AOAC Int 78:1502–1512

    CAS  Google Scholar 

  25. Ueno E, Oshima H, Saito I, Matsumoto H (2001) J Food Hyg Soc Jpn 42:385–393

    Article  CAS  Google Scholar 

  26. Jover E, Bayona JM (2002) J Chromatogr A 950:213–220. doi:10.1016/S0021-9673(02)00019-5

    Article  CAS  Google Scholar 

  27. Barrek S, Paisse O (2003) Anal Bioanal Chem 376:355–359

    CAS  Google Scholar 

  28. Burke ER, Holden AJ, Shaw IC (2003) Chemosphere 50:529–535. doi:10.1016/S0045-6535(02)00492-7

    Article  CAS  Google Scholar 

  29. Causon R (1998) J Chromatogr B Analyt Technol Biomed Life Sci 689:175–180

    Google Scholar 

  30. Ribani M, Bottoli CBG, Collins CH (2004) Quim Nova 27:771–780. doi:10.1590/S0100-40422004000500017

    CAS  Google Scholar 

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Acknowledgments

We thank CNPq (Brazil) for financial support and CAPES (Brazil) for the fellowship. The authors thank Dr. André de Kok from Food and Consumer Product Safety Authority and Dr. Henk Lingeman from The Vrije Universiteit, Amsterdam (The Netherlands) for their assistance reviewing the manuscript.

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Authors and Affiliations

  1. Laboratório de Análises de Resíduos de Pesticidas, Departmento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Michele Antoniuk Presta, Diana I. Ströher Kolberg, Cristine Wickert, Ionara R. Pizzutti, Martha Bohrer Adaime & Renato Zanella

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  1. Michele Antoniuk Presta
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  2. Diana I. Ströher Kolberg
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  3. Cristine Wickert
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  4. Ionara R. Pizzutti
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  5. Martha Bohrer Adaime
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  6. Renato Zanella
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Correspondence to Renato Zanella.

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Presta, M.A., Kolberg, D.I.S., Wickert, C. et al. High Resolution Gel Permeation Chromatography Followed by GC–ECD for the Determination of Pesticide Residues in Soybeans. Chroma 69, 237–241 (2009). https://doi.org/10.1365/s10337-008-0896-8

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