Abstract
Simultaneous derivatization and dispersive liquid–liquid microextraction technique for gas chromatographic determination of fatty acids in water samples is presented. One hundred microlitre of ethanol:pyridine (4:1) were added to 4 mL aqueous sample. Then a solution containing 0.960 mL of acetone (disperser solvent), 10 μL of carbon tetrachloride (extraction solvent) and 30 μL of ethyl chloroformate (derivatization reagent) were rapidly injected into the aqueous sample. After centrifugation, 1 μL sedimented phase with the analytes was analyzed by gas chromatography. The effects of extraction solvent type, derivatization, extraction, and disperser solvents volume, extraction time were investigated. The calibration graphs were linear up to 10 mg L−1 for azelaic acid (R 2 = 0.998) and up to 1 mg L−1 for palmitic and stearic acids (R 2 = 0.997). The detection limits were 14.5, 0.67 and 1.06 μg L−1 for azelaic, palmitic, and stearic acids, respectively. Repeatabilities of the results were acceptable with relative standard deviations (RSD) up to 13%. A possibility to apply the proposed method for fatty acids determination in tap, lake, sea, and river water was demonstrated.
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Wu J, Lee HK (2008) J Chromatogr A 1133:13–20. doi:10.1016/j.chroma.2006.08.006
Rigol A, Latorre A, Lacorte S, Barceló D (2002) J Chromatogr A 963:265–275. doi:10.1016/S0021-9673(02)00232-7
Del Río JC, Romero J, Gutiérrez A (2000) J Chromatogr A 874:235–245. doi:10.1016/S0021-9673(00)00111-4
Pan L, Pawliszyn J (1997) Anal Chem 69:196–205. doi:10.1021/ac9606362
Rigol A, Latorre A, Lacorte S, Barcelo D (2003) J Mass Spectrom 38:417–426. doi:10.1002/jms.454
Rosenfeld JM (2002) Anal Chim Acta 465:93–100. doi:10.1016/S0003-2670(02)00467-1
Seppanen-Laakso T, Laakso I, Hiltunen R (2002) Anal Chi Acta 465:39–62
Brondz I (2002) Ana Chim Acta 465:1–37. doi:10.1016/S0003-2670(01)01467-2
Sobolevsky TG, Revelsky AI, Revelsky IA, Miller B, Oriedo V (2003) J Chromatogr B Analyt Technol Biomed Life Sci 800:101–107. doi:10.1016/j.jchromb.2003.09.013
Peris-Vicente J, Adelantado JVG, Carbo MTD, Castro RM, Reig FB (2006) J Chromatog A 1101:254–260
Husek P (1998) J Chromatogr B Analyt Technol Biomed Life Sci 717:57–91. doi:10.1016/S0378-4347(98)00136-4
Liu Y, Cho SR, Danielson ND (2002) Anal Bioanal Chem 373:64. doi:10.1007/s00216-002-1271-5
Tomaino RM, Parker JD, Larick DK J Agr Food Chem 49: 3993–3998
Pan L, Adams M, Pawliszyn J (1995) Anal Chem 67:4396–4403. doi:10.1021/ac00119a031
Panavaite D, Adomaviciute E, Vickackaite V (2006) Chemija 17:61–66
Tan L, Zhao XP, Liu XQ, Ju HX, Li JS (2005) Chromatographia 62:305–309. doi:10.1365/s10337-005-0604-x
Zhao GH, Liu JF, Nyman M, Jonsson JA (2007) J Chromatogr B Analyt Technol Biomed Life Sci 846:202–208. doi:10.1016/j.jchromb.2006.09.027
Rezaee M, Assadi Y, Milani Hosseini MR, Aghaee E, Ahmadi F, Berijani S (2006) J Chromatogr A 1116:1–9. doi:10.1016/j.chroma.2006.03.007
Zhao E, Zhao W, Han L, Jian S, Zhou Z (2007) J Chromatogr A 1175:137–140. doi:10.1016/j.chroma.2007.10.069
Farina L, Boido E, Carrau F, Dellacassa E (2007) J Chromatogr A 1157:46–50. doi:10.1016/j.chroma.2007.05.006
Fattahi N, Samadi S, Assadi Y, Milani Hosseini MR (2007) J Chromatogr A 1169:63–69. doi:10.1016/j.chroma.2007.09.002
Liang P, Xu J, Li Q (2008) Anal Chim Acta 609:53–58. doi:10.1016/j.aca.2007.12.025
Farahani H, Norouzi P, Dinarvand R, Ganjali MR (2007) J Chromatogr A 1172:105–112. doi:10.1016/j.chroma.2007.10.001
Nagaraju D, Huang SD (2007) J Chromatogr A 1161:89–97. doi:10.1016/j.chroma.2007.05.065
Fattahi N, Assadi Y, Milani Hosseini MR, Zeini Jahromi E (2007) J Chromatogr A 1157:25–29. doi:10.1016/j.chroma.2007.04.062
Chiang JS, Huang SD (2008) Talanta 75:70–75. doi:10.1016/j.talanta.2007.10.036
Naseri MT, Milani Hosseini MR, Assadi Y, Kiani A (2008) Talanta 75:26–62. doi:10.1016/j.talanta.2007.10.029
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This work has been supported by the Lithuanian State Science and Studies Foundation (contract T-23/08).
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Pusvaskiene, E., Januskevic, B., Prichodko, A. et al. Simultaneous Derivatization and Dispersive Liquid–Liquid Microextraction for Fatty Acid GC Determination in Water. Chroma 69, 271–276 (2009). https://doi.org/10.1365/s10337-008-0885-y
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DOI: https://doi.org/10.1365/s10337-008-0885-y