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Determination of Piroxicam and Degradation Products in Drugs by TLC

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Abstract

A thin-layer chromatography method with densitometric detection for the determination of piroxicam and its degradation products is described. Separation was on silica gel TLC plates with ethyl acetate/toluene/butylamine (2+2+1, v/v/v) as the mobile phase. Densitometric detection was carried out at 360 nm. The method has high sensitivity, and satisfactory recovery. Based on 1H NMR and LC–MS–MS, it was found that piroxicam decomposes to produce pyridine-2-amine and 2-methyl-2,3-dihydro-4H-1λ6,2-benzotiazin-1,1,4-trione.

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Authors and Affiliations

  1. Department of Inorganic and Analytical Chemistry, Collegium Medicum, Jagiellonian University, 9 Medyczna Str, 30-688, Kraków, Poland

    Małgorzata Starek & Jan Krzek

  2. Department of Organic Chemistry, Collegium Medicum, Jagiellonian University, 9 Medyczna Str, 30-688, Kraków, Poland

    Monika Tarsa & Marek Żylewski

Authors
  1. Małgorzata Starek
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  2. Jan Krzek
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  3. Monika Tarsa
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  4. Marek Żylewski
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Correspondence to Małgorzata Starek.

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Starek, M., Krzek, J., Tarsa, M. et al. Determination of Piroxicam and Degradation Products in Drugs by TLC. Chroma 69, 351–356 (2009). https://doi.org/10.1365/s10337-008-0883-0

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  • DOI: https://doi.org/10.1365/s10337-008-0883-0

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