Skip to main content
SpringerLink
Log in

Simultaneous Nonchiral Determination of Artemisinin and Arteannuin B in Artemisia annua Using Circular Dichroism Detection

  • Limited Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A new method was performed using on-line coupling of nonchiral reversed-phase liquid chromatography (RP-LC) to circular dichroism (CD) spectroscopy for simultaneous determination of artemisinin and arteannuin B in crude plant extracts of Artemisia annua. Analysis was carried out on an LC–CD system equipped with an Agilent TC-C18 column (4.6 mm I.D. × 200 mm L, 5 μm) using gradient of acetonitrile. The method was validated to be practicable and reliable at alterable wavelength in the ranges of 220–420 nm as desired. LOD and LOQ of artemisinin and arteannuin B were 0.08, 0.26 and 0.31, 1.02 μg mL−1, respectively. It was more sensitive than conventional LC–UV and comparatively cheaper than LC–MS in analysis of TCM.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Li DP, Liang XY, Chen XZ, Chen HS, Wen YX (1995) Guihaia (Guangxi ZhiWu) 15:254–255

    Google Scholar 

  2. Liu SQ, Tian N, Liu ZH, Huang JN, Li J, Ferreira JF (2008) J Chromatogr A (in press). doi:10.1016/j.chroma.2008.02.089

  3. Zhao SS, Zeng MY (1985) Planta Med 51:233–237. doi:10.1055/s-2007-969466

    Article  CAS  Google Scholar 

  4. Elsohly HN, Croom EM, Elsohly MA (1987) Pharm Res 4:258–260. doi:10.1023/A:1016472531527

    Article  CAS  Google Scholar 

  5. Avery BA, Venkatesh KK, Avery MA (1999) J Chromatogr B Analyt Technol Biomed Life Sci 730:71–80. doi:10.1016/S0378-4347(99)00185-1

    Article  CAS  Google Scholar 

  6. Christen P, Veuthey JL (2001) Curr Med Chem 8:1827–1839

    CAS  Google Scholar 

  7. Peng CA, Ferreira JF, Wood AJ (2006) J Chromatogr A 1133:254–258. doi:10.1016/j.chroma.2006.08.043

    Article  CAS  Google Scholar 

  8. Liu CZ, Zhou HY, Zhao Y (2007) Anal Chim Acta 581:298–302. doi:10.1016/j.aca.2006.08.038

    Article  CAS  Google Scholar 

  9. Acton N, Klayman DL, Rollman IJ (1985) Planta Med 5:445–446. doi:10.1055/s-2007-969545

    Article  Google Scholar 

  10. Bangchang KN, Congpuong K, Hung LN, Molunto P, Karbwang J (1998) J Chromatogr B Analyt Technol Biomed Life Sci 708:201–207. doi:10.1016/S0378-4347(97)00667-1

    Article  Google Scholar 

  11. Chen YQ, Chen HL, Fan LY (2004) Anal Chim Acta 525:239–245. doi:10.1016/j.aca.2004.08.014

    Article  Google Scholar 

  12. Bringmann G, Messer K, Wohlfarth M, Kraus J, Dumbuya K, Rückert M (1999) Anal Chem 71:2678–2686. doi:10.1021/ac990001d

    Article  CAS  Google Scholar 

  13. Ferrari J, Terreaux C, Kurtán T, Szikszai-Kiss A, Antus S, Msonthi JD et al (2003) Helv Chim Acta 86:2768–2778. doi:10.1002/hlca.200390226

    Article  CAS  Google Scholar 

  14. Bringmann G, Feineis D, God R, Maksimenka K, Mühlbacher J, Messer K et al (2004) Tetrahedron 60:8143–8151. doi:10.1016/j.tet.2004.06.093

    Article  CAS  Google Scholar 

  15. Shen CY, Li Y (1991) Acta Chimi Sin 49:183–186

    CAS  Google Scholar 

  16. Shen CY (1992) Fenxi Huaxue 20:1150–1152 (Chinese Journal of Analytical Chemistry)

    Google Scholar 

  17. Liang XT (1982) Acta Chimi Sin 40:287–288

    CAS  Google Scholar 

  18. Agrawal PK, Vishwakarma RA, Jain DC, Roy R (1991) Phytochemistry 30:3469–3471. doi:10.1016/0031-9422(91)83234-C

    Article  CAS  Google Scholar 

  19. Lommen WJ, Schenk E, Bouwmeester HJ, Verstappen FW (2006) Planta Med 72:336–345. doi:10.1055/s-2005-916202

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 110016, Shenyang, China

    Jing Chen & Jinhui Wang

  2. Research Center for Drug Metabolism, College of Life Science, Jilin University, 130023, Changchun, China

    Jingkai Gu

  3. Horticulture College, Shenyang Agricultural University, 110000, Shenyang, China

    Rui Zhao

  4. School of Pharmacy, Shenyang Pharmaceutical University, 110016, Shenyang, China

    Ronghua Dai

Authors
  1. Jing Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jingkai Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rui Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ronghua Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jinhui Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jinhui Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, J., Gu, J., Zhao, R. et al. Simultaneous Nonchiral Determination of Artemisinin and Arteannuin B in Artemisia annua Using Circular Dichroism Detection. Chroma 69, 361–363 (2009). https://doi.org/10.1365/s10337-008-0880-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-008-0880-3

Keywords

Search

Navigation