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Optimization of the LC Separation of 15 Phenolic Compounds with Plackett–Burman Designs

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Abstract

In this study Plackett–Burman designs have been used to find the optimum conditions for separation of 15 phenolic compounds by LC. The responses used were the minimum resolution between two peaks and the analysis time. The adopted factors and experimental field were organic modifier (MeOH or ACN), percentage of acetic acid in the aqueous phase, flow rate, gradient with ten steps of percentage increase of the organic component of the mobile phase, and ten factors representing the duration of the steps. Use of Plackett–Burman designs achieved separation of the phenolic compounds within 45 min.

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Authors and Affiliations

  1. Département de Chimie, Institut National des Sciences Appliquées et de Technologie, B.P. 676, 1080, Tunis cedex, Tunisia

    Randa Ben Guebila & Fayçal Hellal

Authors
  1. Randa Ben Guebila
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  2. Fayçal Hellal
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Correspondence to Fayçal Hellal.

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Guebila, R.B., Hellal, F. Optimization of the LC Separation of 15 Phenolic Compounds with Plackett–Burman Designs. Chroma 69, 195–198 (2009). https://doi.org/10.1365/s10337-008-0878-x

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  • DOI: https://doi.org/10.1365/s10337-008-0878-x

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