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TLC Determination of Amitriptyline HCl, Trifluoperazine HCl, Risperidone and Alprazolam in Pharmaceutical Products

Abstract

A simple, sensitive, and precise thin layer chromatographic (TLC) method for simultaneous analysis of psychopharmacological drugs like amitriptyline HCl, trifluoperazine HCl, risperidone and alprazolam in their single dosage forms has been developed, validated, and used for determination of the compounds in commercial pharmaceutical products. The TLC separation was carried out on Merck TLC aluminium sheets of silica gel 60 F254 using carbon tetrachloride:acetone:triethylamine (8:2:0.3, v/v/v), as mobile phase. Densitometric measurements of their spots were achieved at 250 nm over the concentration range for amitriptyline HCl (50–1,200 ng spot−1), trifluoperazine HCl (50–1,200 ng spot−1), risperidone (100–2,400 ng spot−1) and alprazolam (25–600 ng spot−1). Limit of detection (LOD) for amitriptyline HCl (20 ng spot−1), trifluoperazine HCl (20 ng spot−1), risperidone (40 ng spot−1) and alprazolam (5 ng spot−1) was obtained. The study showed that TLC was sensitive and selective for determination of amitriptyline HCl, trifluoperazine HCl, risperidone and alprazolam using a single mobile phase. This proposed method is able for simultaneous determination of psychopharmacological drugs and also applicable for analysis of pharmaceutical formulations.

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Correspondence to Sejal K. Patel.

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Patel, S.K., Patel, N.J. TLC Determination of Amitriptyline HCl, Trifluoperazine HCl, Risperidone and Alprazolam in Pharmaceutical Products. Chroma 69, 393–396 (2009). https://doi.org/10.1365/s10337-008-0870-5

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  • DOI: https://doi.org/10.1365/s10337-008-0870-5

Keywords

  • Thin layer chromatography
  • Densitometric measurements
  • Pharmaceutical products
  • Amitriptyline HCl, trifluoperazine HCl, risperidone and alprazolam