Abstract
A high-speed counter-current chromatography system (HPCCC) capable of rapid processing has been employed to separate seven flavonoids from a methanolic extract of the leaves of Oroxylum indicum by a one-step isocratic elution using a chloroform–methanol–water (9.5:10:5) two-phase system. LC, MS and NMR have identified the components from the extract as chrysin, baicalein, baicalein-7-O-glucoside, baicalein-7-O-diglucoside, chrysin-7-O-glucuronide, baicalein-7-O-glucuronide, and a chrysin-diglucoside. Baicalein-7-O-glucuronide and chrysin-7-O-glucuronide have been separated from this plant by HSCCC for the first time. The present study also reports a new chrysin-diglucoside from the leaf extract. The results demonstrate that HSCCC is a powerful separation tool and can contribute to identifying and quantifying plant ingredients.
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Acknowledgments
This work has been supported by the National Natural Science Foundation of China under the grant number NSFC-30572311 and the New Century Excellent Talent Foundation of China under the grant number NCET-05-07889. The Brunel side of this study was undertaken by Y. Yuan while she was participating in a student exchange programme funded by the Brunel Graduate School. YHG and IAS would like to thank West Focus for their support of the Institute’s research on Chinese herbal medicines.
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Yuan, Y., Hou, W., Tang, M. et al. Separation of Flavonoids from the Leaves of Oroxylum indicum by HSCCC. Chroma 68, 885–892 (2008). https://doi.org/10.1365/s10337-008-0859-0
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DOI: https://doi.org/10.1365/s10337-008-0859-0