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Chromatographia

, 68:955 | Cite as

Quantitative RP–LC Analysis of Mangiferin and Homomangiferin in Mangifera indica L. Leaves and in Mangifera persiciforma C.Y. Wu et T.L. Ming Leaves

  • Jieping QinEmail author
  • Jiagang Deng
  • Xu Feng
  • Qin Wang
  • Shengbo Wang
Original

Abstract

Mangiferin is a pharmacologically active flavonoid, a natural xanthone C-glycoside. Homomangiferin is mangiferin monomethyl ether. Mangiferin and homomangiferin have important medicinal properties and are widely used to relieve many symptoms, for example coughing and asthma. A new gradient reversed-phase high-performance liquid chromatographic method has been developed for analysis of these compounds. The amounts of mangiferin and homomangiferin in 20 samples of Mangifera indica L. leaves and in five samples of M. persiciforma C.Y. Wu et T.L. Ming leaves were quantified by use of the method. The method was validated for system suitability, precision, accuracy, and linearity. The average recovery of the method at three levels was 98.7–101.5% and linearity was good (r > 0.9998) for both mangiferin and homomangiferin over a relatively wide range of concentration. The amounts of the two flavonoid compounds in M. indica L. leaves of different species or collected in different months, and in M. persiciforma C.Y. Wu et T.L. Ming leaves from different collection sites were evaluated in this study and the differences are discussed.

Keywords

Column liquid chromatography Mangiferin Homomangiferin Mangifera indica L. Mangifera persiciforma C.Y. Wu et T.L. Ming 

Notes

Acknowledgments

This study was supported by the project of National Science and Technology Ministry (no. 2004BA901A09) and the Innovation Fund of Guangxi province (no. Guikeneng 0630006-5A).

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Jieping Qin
    • 1
    Email author
  • Jiagang Deng
    • 1
  • Xu Feng
    • 1
  • Qin Wang
    • 1
  • Shengbo Wang
    • 1
  1. 1.Faculty of PharmacyGuangxi Traditional Chinese Medical UniversityNanningPeople’s Republic of China

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