Abstract
Isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate (IDHP), a metabolite of Danshensu, from Salvia miltiorrhiza, has been proved to have potential as a novel drug for regulation of vasomotor activity in small-resistance vascular circulation. In this presentation we report a new specific method for analysis of IDHP in rabbit plasma. Plasma samples were pretreated with 1.5% formic acid in acetonitrile to remove the protein, and the resulting supernatant was extracted with ethyl acetate. Chromatographic separation was achieved on a C18 column with 15.0% acetonitrile in 0.3% aqueous formic acid (pH 2.2) as mobile phase. Multiple-reaction-mode ion-trap mass spectrometry was selected for accurate analysis of IDHP. The calibration plot was linear in the range 0.1–200.0 ng mL−1 for plasma samples. The detection limit was 0.02 ng mL−1. Intra-day and inter-day coefficients of variation were <13.0% and intra-day and inter-day accuracy was within ±8.0% of known concentrations. Finally, the method was used to investigate the pharmacokinetics of IDHP in rabbits; the results indicated IDHP was eliminated rapidly after oral administration.
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Acknowledgments
This work was supported by grants from the Ministry of Science and Technology of Shaanxi Province (no. 2006kz10-G5), the Shaanxi Natural Fund of Science (2006B17), and the West Light Foundation of The Chinese Academy of Sciences (no. 2007DF02).
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Zhang, Q., Li, Z., Zhao, X. et al. Analysis of Isopropyl 3-(3,4-Dihydroxyphenyl)-2-hydroxypropanoate, a Metabolite of Danshensu, from Salvia miltiorrhiza, in Rabbit Plasma. Chroma 68, 1067–1070 (2008). https://doi.org/10.1365/s10337-008-0840-y
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DOI: https://doi.org/10.1365/s10337-008-0840-y