Skip to main content
SpringerLink
Log in

Determination of Dothiepin in Human Plasma by LC–ESI–MS and its Application to Bioequivalence Studies

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

An LC–MS method for the determination of dothiepin in human plasma was developed and validated. Sample preparation involved extraction with n-hexane:2-propanol (95:5). Separation was on an Ultimate XB C18 column (2.1 × 150 mm, 5 μm). A single-quadrupole mass spectrometer with an electrospray interface was operated in the selected-ion monitoring mode to detect the [M+H]+ ions at m/z 296 for dothiepin and at m/z 278 for the internal standard (amitriptylene). The method demonstrated good linearity from 0.78 ng mL−1 (the LOQ) to100 ng mL−1. The mean extraction recovery was 82.4% for dothiepin and and 84.2% for the internal standard. The intra-day and inter-day precision ranged from 8.5 to 11.4% and 9.7 to 12.1% (RSD), respectively. The method was successfully applied to bioequivalence studies of dothiepin hydrochloride tablets to obtain the pharmacokinetic parameters.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Furlanut M, Benetello P, Spina E (1993) Clin Pharmacokinet 24:301–318

    Article  CAS  Google Scholar 

  2. Donoghue J, Hylan TR (2001) Br J Psychiatry Suppl 42:9–17. doi:10.1192/bjp.179.42.s9

    Article  Google Scholar 

  3. Preskorn SH, Jerkovich GS (1990) J Clin Psychopharmacol 10:88–95

    Article  CAS  Google Scholar 

  4. Bickeboeller-Friedrich J, Maurer HH (2001) Ther Drug Monit 23:61–70. doi:10.1097/00007691-200102000-00012

    Article  CAS  Google Scholar 

  5. Hackett LP, Dusci LJ, Ilett KF (1998) Ther Drug Monit 20:30–34. doi:10.1097/00007691-199802000-00006

    Article  CAS  Google Scholar 

  6. Tanaka E, Terada M, Nakamura T, Misawa S, Wakasugi C (1997) J Chromatogr B Analyt Technol Biomed Life Sci 692:405–412. doi:10.1016/S0378-4347(96)00510-5

    Article  CAS  Google Scholar 

  7. Frahnert C, Rao ML, Grasmäder K (2003) J Chromatogr B Analyt Technol Biomed Life Sci 794:35–47. doi:10.1016/S1570-0232(03)00393-3

    Article  CAS  Google Scholar 

  8. Pawlak Z, Clark B (1989) J Pharm Biomed Anal 7:1903–1907. doi:10.1016/0731-7085(89)80212-2

    Article  CAS  Google Scholar 

  9. Molero-Monfort M, Escuder-Gilabert L, Villanueva Camanas R, Sagrado S, Medina-Hernadez M (2001) J Chromatogr B Analyt Technol Biomed Life Sci 753:225–236. doi:10.1016/S0378-4347(00)00546-6

    Article  CAS  Google Scholar 

  10. Brodie RR, Chasseaud LF, Crampton EL, Hawkins DR, Risdall PC (1977) J Int Med Res 5:387–390

    CAS  Google Scholar 

  11. Taylor PJ, Charles BG, Norris R, Salm P, Ravenscroft PJ (1992) J Chromatogr A 581:152–155. doi:10.1016/0378-4347(92)80460-8

    Article  CAS  Google Scholar 

  12. Shibanoki S, Imamura Y, Arakawa Y, Ishikawa K (1987) J Chromatogr A 415:365–371

    CAS  Google Scholar 

  13. Kollroser M, Schober C (2002) Ther Drug Monit 24:537–544. doi:10.1097/00007691-200208000-00013

    Article  CAS  Google Scholar 

  14. Shinozuka T, Terada M, Tanaka E (2006) Forensic Sci Int 162:108–112. doi:10.1016/j.forsciint.2006.03.038

    Article  CAS  Google Scholar 

  15. Sauvage FL, Gaulier JM, Lachâtre G, Marquet (2006) Ther Drug Monit 28:123–130. doi:10.1097/01.ftd.0000194026.04483.c3

Download references

Author information

Authors and Affiliations

  1. Mobile Post-doctoral Center of Biology, Department of Urology, Xiangya Hospital, Central South University, 410008, Changsha, Hunan, China

    Xiang Chen

  2. Analytical Testing Center of School of Xiangya Medicine, Central South University, 410078, Changsha, Hunan, China

    Ben-Mei Chen, Shao-Gang Liu, Fu-Liang Denga & Ping Zhou

Authors
  1. Xiang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ben-Mei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shao-Gang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fu-Liang Denga
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ping Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ben-Mei Chen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, X., Chen, BM., Liu, SG. et al. Determination of Dothiepin in Human Plasma by LC–ESI–MS and its Application to Bioequivalence Studies. Chroma 68, 941–947 (2008). https://doi.org/10.1365/s10337-008-0835-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-008-0835-8

Keywords

Search

Navigation