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LC Determination of Helicidum in Beagle Dog Plasma: Study on the Pharmacokinetics of Helicidum Orally Disintegrating Tablets Compared to Conventional Tablets

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Abstract

A sensitive and reproducible liquid chromatographic method with UV detection was described for the determination of helicidum in beagle dog plasma. Sample preparation was accomplished through protein precipitation with 15% perchloric acid and chromatographic separation was performed on a reversed phase C18 column at 30 °C. Mobile phase consisted of a mixture of acetonitrile–water at a flow rate of 1.0 mL min−1. Wavelength was set at 270 nm. The method was applied to a pharmacokinetic study of two formulations of helicidum. No statistical difference in the t 1/2 (AUC0–5, AUC0–∞) between the two formulations were observed. The t max of helicidum ODTs is significantly shorter than that of conventional tablets (0.84 h vs. 1.33 h, P< 0.05).

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Acknowledgments

The project was supported by Yunnan Natural Science Foundation of China (No: 2003C0016R).

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Authors and Affiliations

  1. Kunming General Hospital of Chengdu Military Command, 650032, Kunming, China

    Jian-Chang He, Di Liu, Qing Zhang, En-Fu Feng, Yuan-Yuan Gong & Gui-Li Xu

  2. Key Laboratory of Drug Metabolism and Pharmacokinetic, China Pharmaceutical University, 210009, Nanjing, China

    Sheng-Hua Gu

Authors
  1. Jian-Chang He
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  2. Sheng-Hua Gu
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  3. Di Liu
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  4. Qing Zhang
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  5. En-Fu Feng
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  6. Yuan-Yuan Gong
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  7. Gui-Li Xu
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Corresponding author

Correspondence to Gui-Li Xu.

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He, JC., Gu, SH., Liu, D. et al. LC Determination of Helicidum in Beagle Dog Plasma: Study on the Pharmacokinetics of Helicidum Orally Disintegrating Tablets Compared to Conventional Tablets. Chroma 68, 1009–1012 (2008). https://doi.org/10.1365/s10337-008-0807-z

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  • DOI: https://doi.org/10.1365/s10337-008-0807-z

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