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Simultaneous LC Estimation of Glimepiride and Metformin in Glimepiride Immediate Release and Metformin Sustained Release Tablets

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Abstract

In the present study, a simple, rapid and precise liquid chromatographic method was developed and validated for the simultaneous determination of glimepiride and metformin in sustained release formulation. The separation was achieved on a Nucleosil 100-5SA column, 250 × 4.6 mm i.d., 5 μm using a mobile phase composed of 1.7 % ammonium dihydrogen phosphate buffer pH 3.0: acetonitrile (70:30 v/v).The instrumental settings were a flow rate of 1.0 ml min−1 and a detector wavelength of 230 nm. The retention time of glimepiride and metformin were 5.1 and 11.3, respectively. The developed method was validated in terms of specificity, sensitivity, linearity, range, accuracy, precision and ruggedness. The proposed method was successfully applied to the sustained release pharmaceutical dosage form for the simultaneous determination of glimepiride and metformin without any interference by excipients.

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Authors and Affiliations

  1. Corporate Analytical Development Lab, Ipca Laboratories Ltd, Charkop, Kandivali (W), Mumbai, 400067, India

    Shraddha P. Pawar & Manisha U. Phadke

  2. Department of Chemistry, University of Mumbai, Vidyanagari, Santa Cruz (E), Mumbai, 400098, India

    Gangadhar A. Meshram

Authors
  1. Shraddha P. Pawar
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  2. Gangadhar A. Meshram
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  3. Manisha U. Phadke
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Correspondence to Shraddha P. Pawar.

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Pawar, S.P., Meshram, G.A. & Phadke, M.U. Simultaneous LC Estimation of Glimepiride and Metformin in Glimepiride Immediate Release and Metformin Sustained Release Tablets. Chroma 68, 1063–1066 (2008). https://doi.org/10.1365/s10337-008-0802-4

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  • DOI: https://doi.org/10.1365/s10337-008-0802-4

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