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Quality Assessment of Veratrum nigrum L. by LC-ELSD Fingerprints and LC Quantitative Analysis

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Abstract

A simple, rapid and valid fingerprint method has been first carried out for the quality control of Veratrum nigrum L. (Chinese name: Lilu), by using liquid chromatography (LC) with evaporative light scattering detection (ELSD). A standard fingerprint was constructed from ten batches of Lilu from different geographic locations to evaluate batch-to-batch consistency. Twelve chromatographic peaks were selected as characteristic peaks and their relative peak areas were calculated for quantitative expression of the LC fingerprints. Furthermore, the amounts of three main components (veratrosine, jervine, and veratramine) were determined simultaneously to evaluate and control quality of Lilu.

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Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (No. 40706046), Knowledge Innovation Program of Chinese Academy of Sciences (LYQY200703), and Guangdong Key Laboratory of Marine Materia Medica Foundation.

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Authors and Affiliations

  1. Jilin Provincial Academy of Chinese Medicine Sciences, 130-021, Changchun, China

    Lishu Wang

  2. Changchun University of Chinese Medicine, 130-117, Changchun, China

    Lishu Wang & Daqing Zhao

  3. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 510-301, Guangzhou, China

    Yonghong Liu

Authors
  1. Lishu Wang
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  2. Daqing Zhao
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  3. Yonghong Liu
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Correspondence to Yonghong Liu.

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Wang, L., Zhao, D. & Liu, Y. Quality Assessment of Veratrum nigrum L. by LC-ELSD Fingerprints and LC Quantitative Analysis. Chroma 68, 961–967 (2008). https://doi.org/10.1365/s10337-008-0799-8

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