Determination of trans-Resveratrol in Bio-Matrices for in Vitro, ex Vivo, and in Vivo Pharmacokinetic Studies by LC Spectrometric Analysis

Abstract

A simple and reproducible liquid chromatographic method was developed for analyzing trans-resveratrol (TR) in cell suspension, intestinal Krebs’ buffer and rat plasma. TR and internal standard (IS, caffeine) were extracted by simple liquid–liquid extraction with acetonitrile. A chromatographic separation of TR and IS was achieved by Hypersil ODS₂ C₁₈ column using the mobile phase consisting of a mixture of methanol and distilled water with approximate retention times of 5.5 and 3.4 min, respectively. The detector wavelength was 303 nm. The limit of quantifications in cell suspension, Krebs’ buffer, and rat plasma were 0.10 μM, 0.05 μg mL⁻¹, and 0.05 μg mL⁻¹. The coefficients of correlation were better than 0.9995 in all solvents. The recovery of TR in the three bio-samples ranged from 86.64 to 102.4%. Intra-day and inter-day accuracy were in the range 0.55–11.50%. The proposed method was successfully applied to Caco-2 cells, everted gut sac and rat pharmacokinetic studies. Among the pharmacokinetic data obtained, TR was concentration-dependent uptaken by Caco-2 cells. The colon was the best situation for TR absorption. The absorption of TR after oral administration was rapid, T _1/2 and AUC _0~∞ were 104 min, and 3.49 ± 0.55 min·(μg mL mg)⁻¹, respectively.