Skip to main content
SpringerLink
Log in

A New LC Method for Determination of Some Aminoglycoside Antibiotics in Dosage Forms and Human Plasma Using 7-Fluoro-4-nitrobenz-2-oxa-1,3-diazole as a Fluorogenic Pre-Column Label

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A validated, selective and sensitive pre-column derivatization chromatographic method for determination of some aminoglycoside antibiotics, namely, amikacin (AMK), gentamicin and neomycin sulphates, has been developed. Determination was obtained by pre-column reaction of the samples with 7-fluoro-4-nitrobenz-2-oxa-1,3-diazole and separating the corresponding derivatives on a reversed phase LC column (ultrasphere C18, 250 × 4.6 mm) and fluorescence detection (λ ex 465 nm, λ em 531 nm). Linear quantitative response curve was generated over a concentration range of 0.05–10 μg mL−1 with a correlation coefficient of more than 0.999 with all studied compounds. The accuracy and precisions were satisfactory for determination of all drugs in dosage forms within- and between-run assay. Furthermore, the method was extended to the determination of AMK in spiked human plasma. The method is specific for the intact drugs, and can be adopted in the presence of co-formulated drugs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Sweetman S (ed) (2002) Martindale, the complete drug reference, 33rd edn. Pharmaceutical Press, London

  2. The United States Pharmacopeia (2006) 29, NF 24. The United States Pharmaceutical Convention, Rockville

  3. British pharmacopoeia (2005) vol II. The Stationary Office, London

  4. Preu M, Guyot D, Petz M (1998) J Chromatogr A 818:95. doi:10.1016/S0021-9673(98)00537-8

    Article  CAS  Google Scholar 

  5. Agarwal VK (1990) J Liq Chromatogr 13:2475. doi:10.1080/01483919008049047

    Article  CAS  Google Scholar 

  6. Clarot I, Chaimbault P, Hasdenteufel F, Netter P, Nicolas A (2004) J Chromatogr A 1031:281–287. doi:10.1016/j.chroma.2003.12.032

    Article  CAS  Google Scholar 

  7. Clarot I, Regazzeti A, Auzeil N, Laadani F, Citton M, Netter P et al (2005) J Chromatogr A 1087:236. doi:10.1016/j.chroma.2005.05.054

    Article  CAS  Google Scholar 

  8. Nicoli S, Santi P (2006) J Pharm Biomed Anal 41:994–997. doi:10.1016/j.jpba.2005.12.029

    Article  CAS  Google Scholar 

  9. Tawa R, Matsunaga H, Fujimoto T (1998) J Chromatogr A 812:141–150. doi:10.1016/S0021-9673(98)00342-2

    Article  CAS  Google Scholar 

  10. Imai K, Uzu S, Toyooka T (1994) Anal Chim Acta 290:3–20. doi:10.1016/0003-2670(94)80034-0

    Article  CAS  Google Scholar 

  11. Al-Majed A, Belal F, Abounassif M, Khalil N (2003) Mikrochim Acta 141:1–6. doi:10.1007/s00604-002-0920-x

    CAS  Google Scholar 

  12. Megoulas NC, Koupparis MA (2004) J Pharm Biomed Anal 36:73–79. doi:10.1016/j.jpba.2004.05.018

    Article  CAS  Google Scholar 

  13. Löffler D, Ternes TA (2003) J Chromatogr A 1000:583–588. doi:10.1016/S0021-9673(03)00059-1

    Article  Google Scholar 

  14. Al-Amoud AI, Clark BJ, Chrystyn H (2002) J Chromatogr B Analyt Technol Biomed Life Sci 769:89–95. doi:10.1016/S1570-0232(01)00636-5

    Article  CAS  Google Scholar 

  15. International conference on harmonization (ICH) guidelines (2005) Validation of analytical procedures—text and methodology Q2 (R1)

  16. Belal F, Abdine H, Al-Majed A, Khalil NY (2002) J Pharm Biomed Anal 27:253–260. doi:10.1016/S0731-7085(01)00503-9

    Article  CAS  Google Scholar 

  17. Jezowska-Bojezuk M, Karaczyn A, Bal W (1998) J Chem Soc Dalton Trans 153. doi:10.1039/a704834c

  18. Jezowska-Bojezuk M, Karaczyn A, Bal W (1998) J Inorg Biochem 71:129. doi:10.1016/S0162-0134(98)10044-2

    Article  Google Scholar 

  19. Jezowska-Bojezuk M, Bal W, Kozlowski JH (1998) Inorg Chim Acta 275–276:541–545. doi:10.1016/S0020-1693(97)06113-6

    Article  Google Scholar 

  20. Serrano JM, Silva M (2006) J Chromatogr A 1117:176–183. doi:10.1016/j.chroma.2006.03.086

    Article  CAS  Google Scholar 

  21. Soltes L (1999) Biomed Chromatogr 13:3–10. doi:10.1002/(SICI)1099-0801(199902)13:1<3::AID-BMC811>3.0.CO;2-T

Download references

Acknowledgments

The author gratefully acknowledges Mr. Nasr Khalil for his technical assistance.

Author information

Authors and Affiliations

  1. Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi Arabia

    Abdulrahman A. Al-Majed

Authors
  1. Abdulrahman A. Al-Majed
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Abdulrahman A. Al-Majed.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Al-Majed, A.A. A New LC Method for Determination of Some Aminoglycoside Antibiotics in Dosage Forms and Human Plasma Using 7-Fluoro-4-nitrobenz-2-oxa-1,3-diazole as a Fluorogenic Pre-Column Label. Chroma 68, 927–934 (2008). https://doi.org/10.1365/s10337-008-0792-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-008-0792-2

Keywords

Search

Navigation