Abstract
Differences in the system constants of the solvation parameter model and retention factor correlation plots for varied solutes are used to study the retention mechanism on XBridge C8, XBridge Phenyl and XTerra Phenyl stationary phases with acetonitrile–water and methanol–water mobile phases containing from 10 to 70% (v/v) organic solvent. These stationary phases are compared with XBridge C18 and XBridge Shield RP18 characterized in an earlier report using the same protocol. The XBridge stationary phases are all quite similar in their retention properties with larger difference in absolute retention explained by differences in cohesion and the phase ratio, mainly, and smaller changes in relative retention (selectivity) by the differences in individual system constants and their variation with mobile phase type and composition. None of the XBridge stationary phases are selectivity equivalent but XBridge C18 and XBridge Shield RP18 have similar separation properties, likewise so do XBridge C8 and XBridge Phenyl, while the differences between the two groups of two stationary phases is greater than the difference within either group. The limited range of changes in selectivity is demonstrated by the high coefficient of determination (>0.98) for plots of the retention factors for varied compounds on the different XBridge phases with the same mobile phase composition.
Similar content being viewed by others
References
Poole CF (2003) The essence of chromatography. Elsevier, Amsterdam
Cheng Y-F, Walter TH, Alden BA, Gendreau C, Neue UD, Grassi JM et al (2000) LC GC North Am 18:1162–1172
Wyndham KD, O’Gara JE, Walter TH, Glose KH, Lawrence NL, Alden BA et al (2003) Anal Chem 75:6781–6788. doi:10.1021/ac034767w
O’Gara JF, Wyndham KD (2006) J Liq Chromatogr Rel Technol 29:1025–1045. doi:10.1080/10826070600574747
Gritti F, Perdu C, Guiochon G (2008) J Chromatogr A 1180:73–89. doi:10.1016/j.chroma.2007.12.022
Gritti F, Guiochon G (2005) Anal Chem 77:1020–1030. doi:10.1021/ac040163w
Mendez A, Bosch E, Roses M, Neue UD (2003) J Chromatogr A 986:33–44. doi:10.1016/S0021-9673(02)01899-X
Davies NH, Euerby MR, McCalley DV (2008) J Chromatogr A 1178:71–78. doi:10.1016/j.chroma.2007.11.040
Davies NH, Euerby MR, McCalley DV (2006) J Chromatogr A 1119:11–19. doi:10.1016/j.chroma.2005.11.096
Kiridena W, DeKay C, Villiere ND, Koziol WW, Poole CF (2005) Chromatographia 61:587–593. doi:10.1365/s10337-005-0559-y
Kiridena W, Poole CF, Atapattu SN, Qian J, Koziol WW (2007) Chromatographia 66:453–460. doi:10.1365/s10337-007-0355-y
Vitha M, Carr PW (2006) J Chromatogr A 1126:143–194. doi:10.1016/j.chroma.2006.06.074
Abraham MH, Ibrahim A, Zissimos AM (2004) J Chromatogr A 1037:20–47. doi:10.1016/j.chroma.2003.12.004
Poole CF, Poole SK (2002) J Chromatogr A 965:263–299. doi:10.1016/S0021-9673(01)01361-9
Poole CF, Poole SK, Gunatilleka (2000) Adv Chromatogr 40:159–230
Chu Y, Poole CF (2003) J Chromatogr A 1003:113–121. doi:10.1016/S0021-9673(03)00845-8
Kiridena W, Atapattu S, Poole CF, Koziol WW (2008) Chromatographia 68:11–17. doi:10.1365/s10337-008-0644-0
Lazaro E, Rafols C, Roses M (2005) J Chromatogr A 1081:163–173. doi:10.1016/j.chroma.2005.05.048
Lazaro E, Rafols C, Abraham MH, Roses M (2006) J Med Chem 49:4861–4870. doi:10.1021/jm0602108
Snyder LR, Dolan JW, Carr PW (2004) J Chromatogr A 1060:77–116
Poole CF, Ahmed H, Kiridena W, DeKay C, Koziol WW (2005) Chromatographia 62:553–561. doi:10.1365/s10337-005-0654-0
Kiridena W, DeKay C, Koziol WW, Ali Z, Ahmed H, Poole CF (2006) Chromatographia 63:407–417. doi:10.1365/s10337-006-0794-x
Poole CF, Kiridena W, DeKay C, Koziol WW, Rosencrans RD (2006) J Chromatogr A 1115:133–141. doi:10.1016/j.chroma.2006.02.089
Poole CF, Ahmed H, Kiridena W, DeKay C, Koziol WW (2007) Chromatographia 65:127–139. doi:10.1365/s10337-006-0131-4
Bolliet D, Poole CF, Roses M (1998) Anal Chim Acta 368:129–140. doi:10.1016/S0003-2670(98)00190-1
Roses M, Bolliet D, Poole CF (1998) J Chromatogr A 829:29–40. doi:10.1016/S0021-9673(98)00746-8
Roses M, Oumada FZ, Bosch E (2001) J Chromatogr A 910:187–194. doi:10.1016/S0021-9673(00)01192-4
Abraham MH, Zhao YH (2005) Phys Chem Chem Phys 7:2418–2422. doi:10.1039/b502058a
Abraham MH, Zhao YH (2005) J Org Chem 70:2633–2640. doi:10.1021/jo048078b
Zhao JH, Carr PW (1999) Anal Chem 71:2623–2632. doi:10.1021/ac981321k
Abraham MH, Martins F (2004) J Pharm Sci 93:1508–1523. doi:10.1002/jps.20070
Lazaro E, Rafols C, Abraham MH, Roses M (2006) J Med Chem 49:4861–4870. doi:10.1021/jm0602108
Poole SK, Poole CF (2008) J Sep Sci 31:1118–1123. doi:10.1002/jssc.200700546
Zhao JH, Carr PW (1998) Anal Chem 70:3619–3628
Reta M, Carr PW, Sadek PC, Rutan SC (1999) Anal Chem 71:3484–3496. doi:10.1021/ac990081l
Poole CF, Gunatilleka AD, Sethuraman R (2000) J Chromatogr A 885:17–39. doi:10.1016/S0021-9673(00)00224-7
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kiridena, W., Atapattu, S.N., Poole, C.F. et al. Comparison of the Separation Characteristics of the Organic–Inorganic Hybrid Stationary Phases XBridge C8 and Phenyl and XTerra Phenyl in RP-LC. Chroma 68, 491–500 (2008). https://doi.org/10.1365/s10337-008-0778-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-008-0778-0