Assay of the Related Compounds Thiamphenicol, Florphenicol, and Chloramphenicol by CE

Abstract

The purpose of this study was to examine the migration behaviour of a mixture of thiamphenicol, its two analogues florphenicol and chloramphenicol, and four impurities found in thiamphenicol. Capillary zone electrophoresis was performed with phosphate–borate or borate running buffers, pH 8.5, with or without addition of sodium dodecylsulphate (SDS). Although the compounds could not be resolved in the absence of SDS, supplementation of both types of buffer, at concentrations of 100 mM and higher, with 50 mM SDS enabled separation. On the basis of migration time and resolution, 100 mM borate buffer containing 50 mM SDS was identified as the best for the study. The accuracy and precision of the method were good under these conditions.