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Development and Validation of an Accurate LC Method for the Quantitative Determination of Voriconazole in a New Emulsion Formulation

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Abstract

A simple, sensitive and accurate liquid chromatographic method with UV detection was developed and validated to determine voriconazole in a new emulsion formulation. Chromatographic separation was achieved on a Diamonsil C18 column (250 × 4.6 mm I.D., 5 μm) using a mobile phase consisting of acetonitrile-water-acetic acid (40:60:0.25, v/v/v) at a flow rate of 1.0 mL min−1. The UV detection wavelength was set at 256 nm. The linear calibration curves were obtained in the concentration range of 1.00–100 μg mL−1 with the limit of quantification of 1.00 μg mL−1. The within- and between-run precisions in terms of percentage relative standard deviation were lower than 7.4 and 7.1%, respectively. The accuracy in terms of percentage relative error ranged from −1.5 to 1.4%. This validated method was successfully applied to the determination of the content of voriconazole in a new emulsion formulation.

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Authors and Affiliations

  1. College of Life and Environment, Central University for Nationalities, Beijing, China

    Xiulan Huang

  2. College of Chemistry and Molecular Engineering, Peking University, Beijing, China

    Feng Qiu

  3. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, 100069, Beijing, China

    Shan Cheng

Authors
  1. Xiulan Huang
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  2. Feng Qiu
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  3. Shan Cheng
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Corresponding author

Correspondence to Shan Cheng.

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Huang, X., Qiu, F. & Cheng, S. Development and Validation of an Accurate LC Method for the Quantitative Determination of Voriconazole in a New Emulsion Formulation. Chroma 68, 649–652 (2008). https://doi.org/10.1365/s10337-008-0737-9

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  • DOI: https://doi.org/10.1365/s10337-008-0737-9

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